Unified platform for genetic and serological detection of COVID-19 with single-molecule technology.

Authors :: Furth N
Shilo S
Cohen N
Erez N
Fedyuk V
Schrager AM
Weinberger A
Dror AA
Zigron A
Shehadeh M
Sela E
Srouji S
Amit S
Levy I
Segal E
Dahan R
Jones D
Douek DC
Shema E
Source :: PloS one [PLoS One] 2021 Jul 26; Vol. 16 (7), pp. e0255096. Date of Electronic Publication: 2021 Jul 26 (Print Publication: 2021).
Publication Year :: 2021
Abstract: The COVID-19 pandemic raises the need for diverse diagnostic approaches to rapidly detect different stages of viral infection. The flexible and quantitative nature of single-molecule imaging technology renders it optimal for development of new diagnostic tools. Here we present a proof-of-concept for a single-molecule based, enzyme-free assay for detection of SARS-CoV-2. The unified platform we developed allows direct detection of the viral genetic material from patients' samples, as well as their immune response consisting of IgG and IgM antibodies. Thus, it establishes a platform for diagnostics of COVID-19, which could also be adjusted to diagnose additional pathogens.<br />Competing Interests: Authors declare no competing interests.

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