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Platelet lysate converts M (IFNγ+LPS) macrophages in CD206 + TGF-β + arginase + M2-like macrophages that affect fibroblast activity and T lymphocyte migration.
- Source :
-
Journal of tissue engineering and regenerative medicine [J Tissue Eng Regen Med] 2021 Sep; Vol. 15 (9), pp. 788-797. Date of Electronic Publication: 2021 Jul 31. - Publication Year :
- 2021
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Abstract
- Macrophages, thanks to their extreme plasticity, exert critical roles in wound healing by orchestrating tissue defenses in the early inflammatory phase, and by promoting tissue regeneration and angiogenesis at a later time point. In parallel, platelets release a large number of preformed molecules that could affect immunocyte functions. Platelet-rich plasma and platelet lysate (PL) have been widely used as a therapeutic preside for ulcers, although little is known about the effects of platelet-derived biomolecules on macrophage functions during wound healing. In this study, we analyze the effects of PL on macrophages phenotype and functions. Monocyte-derived macrophages were cultured in the presence of interferon-γ and lipopolysaccharides to induce the M1 polarization and were further exposed to 10% PL. PL treatment reduced CD80, CD86, and PDL-1 and enhanced CD206 and CD200R expression on macrophages analyzed by cytofluorimetry. Additionally, macrophage cultures show reduced TNF-α and CXCL10, while increased arginase protein, PPAR, TGF-β, and VEGF. TGF-β secretion was paralleled by the decrease of NFkB and increase of STAT3, STAT6, and SMAD2 and SMAD4. Supernatants of PL-treated macrophages induced a significant increase of type-I collagen and to a lesser extent of type-III collagen production by fibroblasts. Finally, the supernatant of PL-treated macrophages showed significantly reduced capacity to induce the in vitro migration of T lymphocytes. Our results demonstrate that PL dampens the macrophage secretion of pro-inflammatory cytokines and induces the release of arginase, TGF-β, and VEGF that may affect angiogenesis and tissue regeneration, thus facilitating the wound healing process.<br /> (© 2021 John Wiley & Sons Ltd.)
- Subjects :
- Arginase metabolism
Chemokine CXCL10 metabolism
Collagen Type I metabolism
Collagen Type III metabolism
Fibroblasts drug effects
Humans
Macrophages drug effects
Mannose Receptor metabolism
Phenotype
Signal Transduction drug effects
T-Lymphocytes drug effects
Blood Platelets chemistry
Cell Movement drug effects
Fibroblasts metabolism
Interferon-gamma pharmacology
Lipopolysaccharides pharmacology
Macrophages metabolism
T-Lymphocytes cytology
Transforming Growth Factor beta metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1932-7005
- Volume :
- 15
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Journal of tissue engineering and regenerative medicine
- Publication Type :
- Academic Journal
- Accession number :
- 34311512
- Full Text :
- https://doi.org/10.1002/term.3229