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The blood flow-klf6a-tagln2 axis drives vessel pruning in zebrafish by regulating endothelial cell rearrangement and actin cytoskeleton dynamics.
- Source :
-
PLoS genetics [PLoS Genet] 2021 Jul 28; Vol. 17 (7), pp. e1009690. Date of Electronic Publication: 2021 Jul 28 (Print Publication: 2021). - Publication Year :
- 2021
-
Abstract
- Recent studies have focused on capillary pruning in various organs and species. However, the way in which large-diameter vessels are pruned remains unclear. Here we show that pruning of the zebrafish caudal vein (CV) from ventral capillaries of the CV plexus in different transgenic embryos is driven by endothelial cell (EC) rearrangement, which involves EC nucleus migration, junction remodeling, and actin cytoskeleton remodeling. Further observation reveals a growing difference in blood flow velocity between the two vessels in CV pruning in zebrafish embryos. With this model, we identify the critical role of Kruppel-like factor 6a (klf6a) in CV pruning. Disruption of klf6a functioning impairs CV pruning in zebrafish. klf6a is required for EC nucleus migration, junction remodeling, and actin cytoskeleton dynamics in zebrafish embryos. Moreover, actin-related protein transgelin 2 (tagln2) is a direct downstream target of klf6a in CV pruning in zebrafish embryos. Together these results demonstrate that the klf6a-tagln2 axis regulates CV pruning by promoting EC rearrangement.<br />Competing Interests: The authors have declared that no competing interests exist.
- Subjects :
- Actin Cytoskeleton metabolism
Actin Cytoskeleton physiology
Animals
Animals, Genetically Modified
Capillaries metabolism
Cell Movement
Endothelial Cells metabolism
Endothelial Cells physiology
Kruppel-Like Transcription Factors genetics
Microfilament Proteins genetics
Microfilament Proteins metabolism
Morphogenesis
Muscle Proteins genetics
Muscle Proteins metabolism
Nerve Tissue Proteins metabolism
Zebrafish metabolism
Zebrafish physiology
Zebrafish Proteins metabolism
Blood Circulation physiology
Microfilament Proteins physiology
Muscle Proteins physiology
Nerve Tissue Proteins physiology
Zebrafish Proteins physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1553-7404
- Volume :
- 17
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- PLoS genetics
- Publication Type :
- Academic Journal
- Accession number :
- 34319989
- Full Text :
- https://doi.org/10.1371/journal.pgen.1009690