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Analysis and Construction of a Competitive Endogenous RNA Regulatory Network of Baicalin-Induced Apoptosis in Human Osteosarcoma Cells.
- Source :
-
BioMed research international [Biomed Res Int] 2021 Jul 19; Vol. 2021, pp. 9984112. Date of Electronic Publication: 2021 Jul 19 (Print Publication: 2021). - Publication Year :
- 2021
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Abstract
- Background: Baicalin is an extract from the traditional Chinese herb Scutellaria baicalensis and has the potential to treat osteosarcoma (OS). However, the transcriptome-level mechanism of baicalin-mediated antitumor effects in OS has not yet been investigated. The aim of this study was to analyze the competitive endogenous RNA (ceRNA) regulatory network involved in baicalin-induced apoptosis of OS cells.<br />Methods: In this study, CCK-8 and flow cytometry assays were used to detect the antitumor effects of baicalin on human OS MG63 cells. Furthermore, transcriptome sequencing was employed to establish the long noncoding RNA (lncRNA), microRNA (miRNA), and mRNA profiles.<br />Results: Baicalin inhibited MG63 cell proliferation and induced apoptosis. Totals of 58 lncRNAs, 31 miRNAs, and 2136 mRNAs in the baicalin-treated MG63 cells were identified as differentially expressed RNAs compared to those in control cells. Of these, 2 lncRNAs, 3 miRNAs, and 18 mRNAs were included in the ceRNA regulatory network. The differentially expressed RNAs were confirmed by quantitative real-time PCR (qRT-PCR).<br />Conclusions: By identifying the ceRNA network, our results provide new information about the possible molecular basis of baicalin, which has potential applications in OS treatment.<br />Competing Interests: The authors declare that there are no conflicts of interest.<br /> (Copyright © 2021 Haifeng Lan et al.)
- Subjects :
- Apoptosis drug effects
Cell Line, Tumor
Cell Survival drug effects
Cell Survival genetics
Gene Expression Profiling
Gene Expression Regulation, Neoplastic drug effects
Gene Ontology
Humans
MicroRNAs genetics
MicroRNAs metabolism
Protein Interaction Maps genetics
RNA, Long Noncoding genetics
RNA, Long Noncoding metabolism
RNA, Messenger genetics
RNA, Messenger metabolism
RNA, Neoplasm metabolism
Reproducibility of Results
Apoptosis genetics
Flavonoids pharmacology
Gene Regulatory Networks
Osteosarcoma genetics
Osteosarcoma pathology
RNA, Neoplasm genetics
Subjects
Details
- Language :
- English
- ISSN :
- 2314-6141
- Volume :
- 2021
- Database :
- MEDLINE
- Journal :
- BioMed research international
- Publication Type :
- Academic Journal
- Accession number :
- 34337069
- Full Text :
- https://doi.org/10.1155/2021/9984112