Bowmanella yangjiangensis sp. nov. and Amphritea pacifica sp. nov., isolated from mariculture fishponds in China.

Four Gram-stain-negative, catalase- and oxidase-positive, rod-shaped and motile strains (Y26, Y57 ^T , ZJ14W ^T and RP18W) were isolated from mariculture fishponds in PR China. Comparisons based on 16S rRNA gene sequences showed that strains Y26 and Y57 ^T share 16S rRNA gene sequence similarities in the range of 95.1-98.5 % with species of the genus Bowmanella , and strains ZJ14W ^T and RP18W share 16S rRNA gene sequence similarities in the range of 96.7 -98.8 % with species of the genus Amphritea , respectively. The genome sizes of strains Y26, Y57 ^T , ZJ14W ^T and RP18W were about 4.85, 5.40, 4.70 and 4.70 Mbp with 49.5, 51.7, 51.2 and 51.3 mol% G+C content, respectively. The calculated pairwise OrthoANIu values among strains Y26, Y57 ^T and species of the genus Bowmanella were in the range of 72.6-83.1 %, but the value between strains Y26 and Y57 ^T was 96.2 %. The pairwise OrthoANIu values among strains ZJ14W ^T , RP18W and other species of the genus Amphritea were all less than 93.9 %, but the value between strains ZJ14W ^T and RP18W was 99.3 %. Q-8 was the major respiratory quinone of strains Y26, Y57 ^T , ZJ14W ^T and RP18W, and the major fatty acids of these strains were all C _{16 : 1} ω7 c , C _{16 : 0} and C _{18 : 1} ω7 c . The predominant polar lipids of strains Y26 and Y57 ^T included phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and phosphatidylinositol, but strains ZJ14W ^T and RP18W only contained phosphatidylethanolamine and phosphatidylglycerol. Combining phenotypic, biochemical and genotypic characteristics, strains Y26 and Y57 ^T should belong to the same species and represent a novel member of the genus Bowmanella , and strains ZJ14W ^T and RP18W should belong to the same species and represent a novel member of the genus Amphritea , for which the names Bowmanella yangjiangensis sp. nov. (type strain Y57 ^T =GDMCC 1.2180 ^T =KCTC 82439 ^T ) and Amphritea pacifica sp. nov. (type strain ZJ14W ^T =GDMCC 1.2203 ^T =KCTC 82438 ^T ) are proposed.