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Effects of Enzymatic Pretreatment of Seeds on the Physicochemical Properties, Bioactive Compounds, and Antioxidant Activity of Pomegranate Seed Oil.
- Source :
-
Molecules (Basel, Switzerland) [Molecules] 2021 Jul 28; Vol. 26 (15). Date of Electronic Publication: 2021 Jul 28. - Publication Year :
- 2021
-
Abstract
- Enzymatic pretreatment of seeds is a novel approach that enhances the health benefits of the extracted oil. The study investigated the influence of the enzymatic pretreatment of seeds on the quality of oil from different pomegranate cultivars. The quality of the ultrasound-assisted (and ethanol-extracted) oil was studied, with respect to the refractive index (RI), yellowness index (YI), conjugated dienes (K232), peroxide value (PV) ρ-anisidine value (AV), total oxidation value (TOTOX), total carotenoid content (TCC), total phenolic compounds (TPC), fatty acid composition, phytosterol composition, ferric reducing antioxidant power (FRAP), and 2.2-diphenyl-1-picryl hydrazyl (DPPH) radical scavenging capacity. The seeds of three different pomegranate cultivars ('Wonderful', 'Herskawitz', and 'Acco') were digested with an equal mixture of Pectinex Ultra SPL, Flavourzyme 100 L, and cellulase crude enzymes, at a concentration, pH, temperature, and time of 1.7%, 4.5, 40 °C, and 5 h, respectively. Enzymatic pretreatment of PS increased oil yield, PV, TPC, TCC, and DPPH radical scavenging capacity, but decreased the YI. The levels of K232, AV and TOTOX, fatty acids, phytosterols, RI, and FRAP, were not significantly affected by enzymatic pretreatment of PS. Principal component analysis (PCA) established that oil extracted from the 'Acco' seed after enzymatic pretreatment had higher yield, TPC, TCC, and DPPH radical scavenging capacity. Therefore, enzyme-pretreated 'Acco' pomegranate fruit seed is a source of quality seed oil with excellent antioxidant properties.
- Subjects :
- Antioxidants chemistry
Antioxidants pharmacology
Biphenyl Compounds antagonists & inhibitors
Carotenoids chemistry
Carotenoids isolation & purification
Carotenoids pharmacology
Ethanol chemistry
Fatty Acids chemistry
Fatty Acids isolation & purification
Fatty Acids pharmacology
Fruit chemistry
Functional Food supply & distribution
Humans
Oxidation-Reduction
Phenols chemistry
Phenols isolation & purification
Phenols pharmacology
Phytosterols chemistry
Phytosterols isolation & purification
Phytosterols pharmacology
Picrates antagonists & inhibitors
Plant Oils chemistry
Principal Component Analysis
Solvents chemistry
Sonication methods
Antioxidants isolation & purification
Hydrolases chemistry
Liquid-Liquid Extraction methods
Plant Oils isolation & purification
Pomegranate chemistry
Seeds chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1420-3049
- Volume :
- 26
- Issue :
- 15
- Database :
- MEDLINE
- Journal :
- Molecules (Basel, Switzerland)
- Publication Type :
- Academic Journal
- Accession number :
- 34361727
- Full Text :
- https://doi.org/10.3390/molecules26154575