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Screening, Cloning, Expression and Characterization of New Alkaline Trehalose Synthase from Pseudomonas monteilii and Its Application for Trehalose Production.
- Source :
-
Journal of microbiology and biotechnology [J Microbiol Biotechnol] 2021 Oct 28; Vol. 31 (10), pp. 1455-1464. - Publication Year :
- 2021
-
Abstract
- Trehalose is a non-reducing disaccharide in increasing demand for applications in food, nutraceutical, and pharmaceutical industries. Single-step trehalose production by trehalose synthase (TreS) using maltose as a starting material is a promising alternative process for industrial application due to its simplicity and cost advantage. Pseudomonas monteilii TBRC 1196 was identified using the developed screening method as a potent strain for TreS production. The TreS gene from P. monteilii TBRC 1196 was first cloned and expressed in Escherichia coli . Purified recombinant trehalose synthase (PmTreS) had a molecular weight of 76 kDa and showed optimal pH and temperature at 9.0 and 40°C, respectively. The enzyme exhibited >90% residual activity under mesophilic condition under a broad pH range of 7-10 for 6 h. Maximum trehalose yield by PmTreS was 68.1% with low yield of glucose (4%) as a byproduct under optimal conditions, equivalent to productivity of 4.5 g/l/h using enzyme loading of 2 mg/g substrate and high concentration maltose solution (100 g/l) in a lab-scale bioreactor. The enzyme represents a potent biocatalyst for energy-saving trehalose production with potential for inhibiting microbial contamination by alkaline condition.
- Subjects :
- Amino Acid Sequence
Bacterial Proteins genetics
Bacterial Proteins metabolism
Cloning, Molecular
Enzyme Stability
Glucose metabolism
Glucosyltransferases genetics
Maltose metabolism
Recombinant Proteins metabolism
Glucosyltransferases metabolism
Pseudomonas enzymology
Trehalose biosynthesis
Subjects
Details
- Language :
- English
- ISSN :
- 1738-8872
- Volume :
- 31
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Journal of microbiology and biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 34409951
- Full Text :
- https://doi.org/10.4014/jmb.2106.06032