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Polyvinyl-Pyrrolidone-Coated Silver Nanoparticles-The Colloidal, Chemical, and Biological Consequences of Steric Stabilization under Biorelevant Conditions.
- Source :
-
International journal of molecular sciences [Int J Mol Sci] 2021 Aug 12; Vol. 22 (16). Date of Electronic Publication: 2021 Aug 12. - Publication Year :
- 2021
-
Abstract
- (1) Background: Several properties of silver nanoparticles (AgNPs), such as cytotoxic, anticancer, and antimicrobial activities, have been subjects of intense research; however, important aspects such as nanoparticle aggregation are generally neglected, although a decline in colloidal stability leads to a loss of the desired biological activities. Colloidal stability is affected by pH, ionic strength, or a plethora of biomolecules that interact with AgNPs under biorelevant conditions. (2) Methods: As only a few studies have focused on the relationship between aggregation behavior and the biological properties of AgNPs, here, we have systematically evaluated this issue by completing a thorough analysis of sterically (via polyvinyl-pyrrolidone (PVP)) stabilized AgNPs that were subjected to different circumstances. We assessed ultraviolet-visible light absorption, dynamic light scattering, zeta potential measurements, in vitro cell viability, and microdilution assays to screen both colloidal stability as well as bioactivity. (3) Results: The results revealed that although PVP provided outstanding biorelevant colloidal stability, the chemical stability of AgNPs could not be maintained completely with this capping material. (4) Conclusion: These unexpected findings led to the realization that stabilizing materials have more profound importance in association with biorelevant applications of nanomaterials than just being simple colloidal stabilizers.
- Subjects :
- Anti-Infective Agents chemistry
Antineoplastic Agents chemistry
Cell Line, Tumor
Cell Survival drug effects
Dynamic Light Scattering
HeLa Cells
Humans
Hydrogen-Ion Concentration
Metal Nanoparticles
Microbial Sensitivity Tests
Microscopy, Electron, Transmission
Silver chemistry
Anti-Infective Agents pharmacology
Antineoplastic Agents pharmacology
Gram-Negative Bacteria drug effects
Gram-Positive Bacteria drug effects
Povidone chemistry
Silver pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1422-0067
- Volume :
- 22
- Issue :
- 16
- Database :
- MEDLINE
- Journal :
- International journal of molecular sciences
- Publication Type :
- Academic Journal
- Accession number :
- 34445378
- Full Text :
- https://doi.org/10.3390/ijms22168673