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Na + -dependent inactivation of vascular Na + /Ca 2+ exchanger responsible for reduced peripheral blood flow in neuropathic pain model.
- Source :
-
European journal of pharmacology [Eur J Pharmacol] 2021 Nov 05; Vol. 910, pp. 174448. Date of Electronic Publication: 2021 Aug 26. - Publication Year :
- 2021
-
Abstract
- Reduced skin blood flow has been reported in neuropathic pain patients as well as various peripheral neuropathic pain model animals. We have previously shown that vasodilators, which improves reduced skin blood flow, correlatively alleviate neuropathic pain in chronic constriction injury (CCI) mice, a model of neuropathic pain from peripheral nerve injury. Here, we sought to elucidate the mechanism underlying the reduced skin blood flow in CCI rats. The skin blood flow of the ipsilateral plantar arteries was significantly reduced compared to that of the contralateral ones 4 weeks after loose ligation of the sciatic nerve. The contraction induced by noradrenaline, serotonin, and U46619, a thromboxane receptor agonist, in the isolated ipsilateral plantar arteries was significantly enhanced compared to that in the contralateral ones. KB-R7943, a Na <superscript>+</superscript> /Ca <superscript>2+</superscript> exchanger (NCX) inhibitor, shifted the concentration-response curves of noradrenaline to the left in the contralateral arteries but had no effect on the ipsilateral side. There was no significant difference in concentration-response curves of noradrenaline between the ipsilateral and contralateral arteries in the presence of KB-R7943. Amiloride, a non-specific inhibitor of Na <superscript>+</superscript> channels and transporters, comparably shifted concentration-response curves of noradrenaline to the left in both the contralateral and ipsilateral arteries. One hundred nM of noradrenaline induced intracellular Ca <superscript>2+</superscript> elevation in the ipsilateral arteries, which was significantly larger than that induced by 300-nM noradrenaline in the contralateral arteries. These results suggest that reduced peripheral blood flow after nerve injury is due to Na <superscript>+</superscript> -dependent inactivation of NCX in the ipsilateral plantar arteries.<br /> (Copyright © 2021 Elsevier B.V. All rights reserved.)
- Subjects :
- 15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid pharmacology
Amiloride pharmacology
Animals
Arteries drug effects
Boron Compounds pharmacology
Calcimycin pharmacology
Calcium metabolism
Calcium Ionophores pharmacology
Disease Models, Animal
Dose-Response Relationship, Drug
Male
Muscle Contraction drug effects
Nifedipine pharmacology
Norepinephrine pharmacology
Ouabain pharmacology
Rats, Wistar
Serotonin pharmacology
Thiourea analogs & derivatives
Thiourea pharmacology
Vasoconstrictor Agents pharmacology
Rats
Blood Circulation drug effects
Neuralgia metabolism
Sodium metabolism
Sodium-Calcium Exchanger antagonists & inhibitors
Sodium-Calcium Exchanger metabolism
Vasodilator Agents pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1879-0712
- Volume :
- 910
- Database :
- MEDLINE
- Journal :
- European journal of pharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 34454926
- Full Text :
- https://doi.org/10.1016/j.ejphar.2021.174448