Back to Search
Start Over
Plasmacytoid Dendritic Cells Facilitate Th Cell Cytokine Responses throughout Schistosoma mansoni Infection.
- Source :
-
ImmunoHorizons [Immunohorizons] 2021 Aug 30; Vol. 5 (8), pp. 721-732. Date of Electronic Publication: 2021 Aug 30. - Publication Year :
- 2021
-
Abstract
- Plasmacytoid dendritic cells (pDCs) are potent producers of type I IFN (IFN-I) during viral infection and respond to IFN-I in a positive feedback loop that promotes their function. IFN-I shapes dendritic cell responses during helminth infection, impacting their ability to support Th2 responses. However, the role of pDCs in type 2 inflammation is unclear. Previous studies have shown that pDCs are dispensable for hepatic or splenic Th2 responses during the early stages of murine infection with the trematode Schistosoma mansoni at the onset of parasite egg laying. However, during S. mansoni infection, an ongoing Th2 response against mature parasite eggs is required to protect the liver and intestine from acute damage and how pDCs participate in immune responses to eggs and adult worms in various tissues beyond acute infection remains unclear. We now show that pDCs are required for optimal Th2 cytokine production in response to S. mansoni eggs in the intestinal-draining mesenteric lymph nodes throughout infection and for egg-specific IFN-γ at later time points of infection. Further, pDC depletion at chronic stages of infection led to increased hepatic and splenic pathology as well as abrogated Th2 cell cytokine production and activation in the liver. In vitro, mesenteric lymph node pDCs supported Th2 cell responses from infection-experienced CD4 <superscript>+</superscript> T cells, a process dependent on pDC IFN-I responsiveness, yet independent of Ag. Together, these data highlight a previously unappreciated role for pDCs and IFN-I in maintaining and reinforcing type 2 immunity in the lymph nodes and inflamed tissue during helminth infection.<br /> (Copyright © 2021 The Authors.)
- Subjects :
- Animals
CD4-Positive T-Lymphocytes immunology
CD4-Positive T-Lymphocytes metabolism
CD4-Positive T-Lymphocytes parasitology
Cytokines metabolism
Dendritic Cells parasitology
Female
Flow Cytometry methods
Host-Parasite Interactions immunology
Lymphocyte Count
Mice, Inbred C57BL
Mice, Knockout
Schistosoma mansoni physiology
Schistosomiasis mansoni metabolism
Schistosomiasis mansoni parasitology
T-Lymphocytes, Helper-Inducer metabolism
T-Lymphocytes, Helper-Inducer parasitology
Th2 Cells immunology
Th2 Cells metabolism
Th2 Cells parasitology
Mice
Cytokines immunology
Dendritic Cells immunology
Lymphocyte Activation immunology
Schistosoma mansoni immunology
Schistosomiasis mansoni immunology
T-Lymphocytes, Helper-Inducer immunology
Subjects
Details
- Language :
- English
- ISSN :
- 2573-7732
- Volume :
- 5
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- ImmunoHorizons
- Publication Type :
- Academic Journal
- Accession number :
- 34462311
- Full Text :
- https://doi.org/10.4049/immunohorizons.2100071