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Shenxiong glucose injection inhibits H 2 O 2 -induced H9c2 cell apoptosis by activating the ERK signaling pathway.
- Source :
-
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie [Biomed Pharmacother] 2021 Nov; Vol. 143, pp. 112114. Date of Electronic Publication: 2021 Aug 30. - Publication Year :
- 2021
-
Abstract
- Background: Shenxiong glucose injection (SGI) is a traditional Chinese medicine injection composed of water extract of Salvia miltiorrhiza and Ligustrazine hydrochloride. SGI has shown strong antioxidant and anti-apoptotic properties. However, the mechanisms underlying its anti-apoptotic effect need to be addressed.<br />Methods: H9c2 cell apoptosis model was established by treatment of hydrogen peroxide (H <subscript>2</subscript> O <subscript>2</subscript> ). Cell survival rates were examined by MTS assay, cell apoptosis rates were determined by flow cytometry, levels of intracellular ROS were assessed by ROS kit, proteome phosphorylation was determined by phosphoproteomic analysis, and extracellular signal-regulated kinase (ERK), phosphorylated ERK, phosphorylated c-Jun, B-cell lymphoma 2 (Bcl-2)-associated X protein (Bax), Bcl-2, and cleaved caspase-3 were examined by Western blot.<br />Result: SGI showed protective effects against H <subscript>2</subscript> O <subscript>2</subscript> -induced reduced cell viability, elevated ROS, and increased apoptosis in H9c2 cells. Phosphorylation proteomics detected a total of 3369 proteins with 78 protein of upregulated phosphorylation and 104 protein of downregulated phosphorylation. Kyoto Encyclopedia Genes and Genomes pathway analyses of differentially phosphorylated proteins showed that the ERK pathway, the downstream pathway of the focal adhesion pathway related to apoptosis, was highly enriched, and the phosphorylation levels of ERK and c-Jun were confirmed by Western blot. In addition, the ERK pathway inhibitor PD98059 significantly inhibited the anti-apoptotic effect of SGI.<br />Conclusion: SGI antagonizes H <subscript>2</subscript> O <subscript>2</subscript> -induced cell apoptosis by activating the ERK pathway.<br /> (Copyright © 2021 The Authors. Published by Elsevier Masson SAS.. All rights reserved.)
- Subjects :
- Animals
Apoptosis Regulatory Proteins metabolism
Cell Line
Hydrogen Peroxide toxicity
Myocytes, Cardiac enzymology
Myocytes, Cardiac pathology
Oxidative Stress drug effects
Phosphorylation
Proteome
Proteomics
Proto-Oncogene Proteins c-jun metabolism
Rats
Signal Transduction
Antioxidants pharmacology
Apoptosis drug effects
Drugs, Chinese Herbal pharmacology
Extracellular Signal-Regulated MAP Kinases metabolism
Myocytes, Cardiac drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1950-6007
- Volume :
- 143
- Database :
- MEDLINE
- Journal :
- Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie
- Publication Type :
- Academic Journal
- Accession number :
- 34474350
- Full Text :
- https://doi.org/10.1016/j.biopha.2021.112114