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Cancer Associated Fibroblasts Derived from Pancreatic Adenocarcinoma and Their Role in Cell Migration.
- Source :
-
Anticancer research [Anticancer Res] 2021 Sep; Vol. 41 (9), pp. 4229-4238. Date of Electronic Publication: 2021 Sep 01. - Publication Year :
- 2021
-
Abstract
- Background/aim: Pancreatic ductal adenocarcinoma (PDAC) shows poor survival and early systemic dissemination. Cancer associated fibroblasts (CAFs) enhance migration and invasion of cancer cells. We aimed to investigate the role of CAFs in cell migration and their underlying paracrine effects.<br />Materials and Methods: Using Transwell <superscript>®</superscript> migration assays, PDAC cells (PANC-1) and three distinct types of fibroblasts were analyzed: CAFs, genetically transformed human foreskin-fibroblasts (BJeLR), and non-transformed human foreskin-fibroblasts (VH7). IL6 in the culture supernatant was measured to investigate paracrine communication in monocultures and direct/indirect cocultures.<br />Results: CAFs showed a significantly higher capacity to migrate in vitro when compared to benign fibroblasts (p=0.009). They also facilitated the migration of PDAC cells in coculture (p=0.001). Neither BJeLR, nor VH7 displayed such features. This was accompanied by a significant increase in IL-6 when CAFs were cocultured with PANC-1 (p=0.009).<br />Conclusion: CAFs are a key element of intra-tumoral migration and should be further investigated as a potential therapeutic target.<br /> (Copyright © 2021 International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.)
- Subjects :
- Cancer-Associated Fibroblasts immunology
Cancer-Associated Fibroblasts pathology
Carcinoma, Pancreatic Ductal immunology
Cell Line, Tumor
Cell Movement
Cell Proliferation
Cells, Cultured
Coculture Techniques
Female
Foreskin immunology
Humans
Male
Middle Aged
Pancreatic Neoplasms immunology
Paracrine Communication
Tumor Microenvironment
Cancer-Associated Fibroblasts cytology
Carcinoma, Pancreatic Ductal pathology
Foreskin cytology
Interleukin-6 metabolism
Pancreatic Neoplasms pathology
Subjects
Details
- Language :
- English
- ISSN :
- 1791-7530
- Volume :
- 41
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Anticancer research
- Publication Type :
- Academic Journal
- Accession number :
- 34475042
- Full Text :
- https://doi.org/10.21873/anticanres.15227