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Assessment of Antimicrobial Activity of Dehydrated Amniotic Membrane in Infectious Keratitis: A Small Retrospective Case Series and In Vitro Study.
- Source :
-
Cornea [Cornea] 2021 Oct 01; Vol. 40 (10), pp. 1348-1352. - Publication Year :
- 2021
-
Abstract
- Purpose: To describe a small case series of infectious keratitis with poor visual outcomes after amniotic membrane (AM) placement and to prospectively evaluate whether AM demonstrates antibacterial activity in vitro against pathogens commonly isolated from infectious corneal ulcers.<br />Methods: A retrospective case series and in vitro study of antibacterial activity of dehydrated AM using disk diffusion and measurement of inhibitory zones for bacterial assessment and inverted microscopy analysis for Acanthamoeba sp. growth.<br />Results: Three cases of known etiology infectious keratitis are described where the clinical presentation worsened after treatment with AM. In vitro analysis of dehydrated AM, with and without a soft contact lens, demonstrated no inhibition of growth against Pseudomonas aeruginosa or Streptococcus pneumoniae. There was minimal growth inhibition of Staphylococcus aureus, although these zones of inhibition were much smaller than that surrounding the positive control. For Acanthamoeba sp., solubilized, dehydrated AM did not alter cyst density.<br />Conclusions: In an in vitro analysis, dehydrated AM did not provide evidence for a potentially clinically meaningful antibacterial effect against organisms commonly isolated from corneal ulcers.<br />Competing Interests: The authors have no conflicts of interest to disclose.<br /> (Copyright © 2020 Wolters Kluwer Health, Inc. All rights reserved.)
- Subjects :
- Acanthamoeba Keratitis parasitology
Acanthamoeba Keratitis surgery
Adolescent
Adult
Amnion transplantation
Anti-Bacterial Agents pharmacology
Eye Infections, Bacterial microbiology
Eye Infections, Bacterial surgery
Humans
Keratitis microbiology
Keratitis surgery
Male
Microbial Sensitivity Tests
Middle Aged
Pseudomonas Infections surgery
Retrospective Studies
Staphylococcal Infections surgery
Streptococcal Infections surgery
Acanthamoeba castellanii drug effects
Amnion microbiology
Amnion parasitology
Moxifloxacin pharmacology
Pseudomonas aeruginosa drug effects
Staphylococcus aureus drug effects
Streptococcus pneumoniae drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1536-4798
- Volume :
- 40
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Cornea
- Publication Type :
- Academic Journal
- Accession number :
- 34481412
- Full Text :
- https://doi.org/10.1097/ICO.0000000000002627