The linear plasmid pSA3239 is essential for the replication of the Streptomyces lavendulae subsp. lavendulae CCM 3239 chromosome.

Authors :: Novakova R
Rückert C
Knirschova R
Feckova L
Busche T
Csolleiova D
Homerova D
Rezuchova B
Javorova R
Sevcikova B
Kalinowski J
Kormanec J
Source :: Research in microbiology [Res Microbiol] 2021 Sep-Oct; Vol. 172 (6), pp. 103870. Date of Electronic Publication: 2021 Sep 03.
Publication Year :: 2021
Abstract: We previously reported the complete genome of Streptomyces lavendulae subsp. lavendulae CCM 3239, containing the linear chromosome and the large linear plasmid pSA3239. Although the chromosome exhibited replication features characteristic for the archetypal end-patching replication, it lacked the tap/tpg gene pair for two proteins essential for this process. However, this archetypal tpgSa-tapSa operon is present in pSA3239. Complete genomic sequence of the S. lavendulae Del-LP strain lacking this plasmid revealed the circularization of its chromosome with a large deletion of both arms. These results suggest an essential role of pSA3239-encoded TapSa/TpgSa in the end-patching replication of the chromosome.<br />Competing Interests: Declaration of competing interest There are no conflicts of interests.<br /> (Copyright © 2021 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.)

Subjects :: Bacterial Proteins genetics
DNA Replication
DNA, Bacterial genetics
Genome, Bacterial
Operon
Bacterial Proteins metabolism
Chromosomes, Bacterial physiology
Plasmids
Streptomyces genetics

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