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Magnetic ZIF-8-Based Mimic Multi-enzyme System as a Colorimetric Biosensor for Detection of Aryloxyphenoxypropionate Herbicides.
- Source :
-
ACS applied materials & interfaces [ACS Appl Mater Interfaces] 2021 Sep 22; Vol. 13 (37), pp. 44329-44338. Date of Electronic Publication: 2021 Sep 08. - Publication Year :
- 2021
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Abstract
- In the present study, a magnetic mimic multi-enzyme system was developed by encapsulating the aryloxyphenoxypropionate (AOPP) herbicide hydrolase QpeH and alcohol oxidase (AOx) in zeolitic imidazolate framework (ZIF-8) nanocrystals with magnetic Fe <subscript>3</subscript> O <subscript>4</subscript> nanoparticles (MNPs) to detect AOPP herbicides. The structural, protein loading capacity and loading ratio, porosity, and magnetic properties of QpeH/AOx@mZIF-8 were characterized by scanning electron microscopy, X-ray diffraction, Fourier transform infrared spectroscopy, thermogravimetric analysis, nitrogen sorption, and vibrating sample magnetometry. An AOPP herbicide colorimetric biosensor made with QpeH/AOx@mZIF-8 had the highest sensitivity toward quizalofop-P-ethyl (QpE) with a limit of detection of 8.2 μM. This system was suitable to detect two other AOPP herbicides, including fenoxaprop-P-ethyl (FpE) and haloxyfop-P-methyl (HpE). The practical application of the biosensor was verified through quantitative analysis of QpE residues in industrial wastewater and field soils. Furthermore, QpeH/AOx@mZIF-8 exhibited excellent long-term storage stability (at least 50 days), easy separation by magnet, and reusability (at least 10 cycles), supporting its promising role in simple and low-cost detection of AOPP herbicides in real environmental samples.
- Subjects :
- Alcohol Oxidoreductases chemistry
Bacterial Proteins chemistry
Carboxylic Ester Hydrolases chemistry
Enzymes, Immobilized chemistry
Herbicides chemistry
Hydrolysis
Limit of Detection
Oxazoles analysis
Oxazoles chemistry
Oxidation-Reduction
Phenyl Ethers chemistry
Propionates chemistry
Pseudomonas enzymology
Quinoxalines analysis
Quinoxalines chemistry
Saccharomycetales enzymology
Biosensing Techniques methods
Colorimetry methods
Herbicides analysis
Metal-Organic Frameworks chemistry
Phenyl Ethers analysis
Propionates analysis
Subjects
Details
- Language :
- English
- ISSN :
- 1944-8252
- Volume :
- 13
- Issue :
- 37
- Database :
- MEDLINE
- Journal :
- ACS applied materials & interfaces
- Publication Type :
- Academic Journal
- Accession number :
- 34494423
- Full Text :
- https://doi.org/10.1021/acsami.1c11815