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Heterologous production of hyaluronic acid in Nicotiana tabacum hairy roots expressing a human hyaluronan synthase 2.
- Source :
-
Scientific reports [Sci Rep] 2021 Sep 09; Vol. 11 (1), pp. 17966. Date of Electronic Publication: 2021 Sep 09. - Publication Year :
- 2021
-
Abstract
- Hyaluronic acid (HA), a unique polysaccharide with excellent Physico-chemical properties, is broadly used in pharmaceutical, biomedical, and cosmetic fields. It is widely present in all vertebrates, certain bacterial strains, and even viruses while it is not found in plants, fungi, and insects. HA is naturally synthesized by a class of integral membrane proteins called Hyaluronic acid synthase (HAS). Thus far, industrial production of HA is carried out based on either extraction from animal sources or large-scale microbial fermentation. The major drawbacks to using these systems are contamination with pathogens and microbial toxins. Recently, the production of HA through recombinant systems has received considerable attention. Plants are eco-friendly ideal expression systems for biopharmaceuticals production. In this study, the optimized human hyaluronic acid synthase2 (hHAS2) sequence was transformed into Nicotiana tabacum using Agrobacterium rhizogenes. The highest rhHAS2 concentration of 65.72 ng/kg (wet weight) in transgenic tobacco hairy roots was measured by the human HAS2 ELISA kit. The HA production in the transgenic hairy roots was verified by scanning electron microscope (SEM) and quantified by the HA ELISA kit. The DPPH radical scavenging activity of HA with the highest concentration of 0.56 g/kg (wet weight) showed a maximum activity of 46%. Gel Permeation Chromatography (GPC) analyses revealed the high molecular weight HA (HMW-HA) with about > 0.8 MDa.<br /> (© 2021. The Author(s).)
- Subjects :
- Agrobacterium genetics
Base Sequence
Biological Products chemistry
Chromatography, Gel methods
Enzyme-Linked Immunosorbent Assay methods
Humans
Hyaluronan Synthases genetics
Hyaluronic Acid chemistry
Microscopy, Electron, Scanning methods
Molecular Weight
Plants, Genetically Modified
Transformation, Genetic
Biological Products metabolism
Hyaluronan Synthases metabolism
Hyaluronic Acid biosynthesis
Plant Roots genetics
Plant Roots metabolism
Nicotiana genetics
Nicotiana metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 11
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 34504153
- Full Text :
- https://doi.org/10.1038/s41598-021-97139-0