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Clinical evaluation of urinary liver-type fatty acid-binding protein for the diagnosis of renal diseases in dogs.
- Source :
-
The Journal of veterinary medical science [J Vet Med Sci] 2021 Sep 27; Vol. 83 (9), pp. 1465-1471. Date of Electronic Publication: 2021 Sep 14. - Publication Year :
- 2021
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Abstract
- Liver-type fatty acid-binding protein (L-FABP) is a biomarker for the early detection of renal diseases in humans. L-FABP is a cytotoxic oxidation product secreted from the proximal tubules under ischemic and oxidative stress conditions. First, L-FABP gene expression in the kidney and liver was evaluated. Next, the urinary L-FABP concentrations in dogs with or without renal diseases were measured using a novel enzyme-linked immunosorbent assay kit. Urinary L-FABP was normalized relative to urinary creatinine (uCre) concentrations (µg/g uCre). Finally, the relationships between urinary L-FABP and renal biomarkers used in canine medicine or serum alanine transaminase (ALT) as an indicator of liver damage were examined. Serum and urine samples from 94 client-owned dogs including 23 dogs with renal diseases and 71 dogs without renal diseases were used for analysis. Relative L-FABP gene expression was confirmed both in the liver and kidney. Dogs with renal diseases had a significantly higher urinary L-FABP than those without, and its predictive cutoff value was 26 µg/g uCre. Urinary L-FABP was significantly correlated with serum creatinine (r=0.4674, P<0.01), urea nitrogen (r=0.4907, P<0.01), urine specific gravity (r=-0.5100, P<0.01), and urine protein/creatinine ratio (r=0.7216, P<0.01), but not with serum ALT. Hence, dogs with a high urinary L-FABP value were more likely to have renal diseases.
Details
- Language :
- English
- ISSN :
- 1347-7439
- Volume :
- 83
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- The Journal of veterinary medical science
- Publication Type :
- Academic Journal
- Accession number :
- 34526412
- Full Text :
- https://doi.org/10.1292/jvms.20-0698