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High prevalence of multidrug-resistant non-fermentative Gram-negative bacilli harboring bla IMP-1 and bla VIM-1 metallo-beta-lactamase genes in Birjand, south-east Iran.
- Source :
-
Iranian journal of microbiology [Iran J Microbiol] 2021 Aug; Vol. 13 (4), pp. 470-479. - Publication Year :
- 2021
-
Abstract
- Background and Objectives: Non-fermentative Gram-negative Bacilli (NFGNB) is known as a major cause of healthcare-associated infections with high levels of antibiotic resistance. The aim of this study was to investigate the antibiotic resistance profiles and molecular characteristics of metallo-beta-lactamase (MBL)-producing NFGNB.<br />Materials and Methods: In this cross-sectional study, the antibiotic resistance profile of 122 clinical NFGNB isolates was determined by the Kirby-Bauer disk diffusion and microdilution broth methods. Bacterial isolates were investigated for the detection of MBLs production using the combination disk diffusion Test (CDDT). The existence of bla <subscript>IMP</subscript> , bla <subscript>VIM</subscript> , and bla <subscript>NDM</subscript> genes in all carbapenem-resistant isolates was determined employing polymerase chain reaction (PCR) assays.<br />Results: High resistance in Pseudomonas aeruginosa was reported to cefotaxime and minocycline, whereas Acinetobacter baumannii isolates were highly resistant to all antibiotics except colistin. Multidrug resistance (MDR)-NFGNB (66% vs. 12.5%, P=0.0004) and extensively drug resistant (XDR)-NFGNB (55.7% vs. 12.5%, P=0.001) isolates were significantly more common in hospitalized patients than in outpatients. The production of MBL was seen in 40% of P. aeruginosa and 93.3% of A. baumannii isolates. It was found that 33.3% and 46.7% of carbapenem-resistant P. aeruginosa isolates, and 13.3% and 28.9% of carbapenem-resistant A. baumannii isolates were harboring bla <subscript>IMP-1</subscript> and bla <subscript>VIM-1</subscript> genes, respectively. The incidence of MDR (98.2% vs. 28.3%, P<0.001) and XDR (96.4% vs. 11.7%, P<0.001) in MBL-producing NFGNB isolates was significantly higher than non-MBL-producing isolates.<br />Conclusion: This study demonstrated a higher rate of resistance among NFGNB isolates with an additional burden of MBL production within them, warranting a need for robust microbiological surveillance and accurate detection of MBL producers among the NFGNB.<br /> (Copyright © 2021 The Authors. Published by Tehran University of Medical Sciences.)
Details
- Language :
- English
- ISSN :
- 2008-3289
- Volume :
- 13
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Iranian journal of microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 34557275
- Full Text :
- https://doi.org/10.18502/ijm.v13i4.6971