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Diversity and function of motile ciliated cell types within ependymal lineages of the zebrafish brain.
- Source :
-
Cell reports [Cell Rep] 2021 Oct 05; Vol. 37 (1), pp. 109775. - Publication Year :
- 2021
-
Abstract
- Motile cilia defects impair cerebrospinal fluid (CSF) flow and can cause brain and spine disorders. The development of ciliated cells, their impact on CSF flow, and their function in brain and axial morphogenesis are not fully understood. We have characterized motile ciliated cells within the zebrafish brain ventricles. We show that the ventricles undergo restructuring through development, involving a transition from mono- to multiciliated cells (MCCs) driven by gmnc. MCCs co-exist with monociliated cells and generate directional flow patterns. These ciliated cells have different developmental origins and are genetically heterogenous with respect to expression of the Foxj1 family of ciliary master regulators. Finally, we show that cilia loss from the tela choroida and choroid plexus or global perturbation of multiciliation does not affect overall brain or spine morphogenesis but results in enlarged ventricles. Our findings establish that motile ciliated cells are generated by complementary and sequential transcriptional programs to support ventricular development.<br />Competing Interests: Declaration of interests N.K., A.G., and B.S.N. are full-time employees of Resolve Biosciences GmbH.<br /> (Copyright © 2021 The Author(s). Published by Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Animals, Genetically Modified metabolism
Brain cytology
Brain pathology
Cell Lineage
Cerebrospinal Fluid physiology
Cilia pathology
Embryo, Nonmammalian metabolism
Ependyma cytology
Ependyma pathology
Forkhead Transcription Factors genetics
Forkhead Transcription Factors metabolism
Gene Editing
Morphogenesis
Nuclear Proteins genetics
Nuclear Proteins metabolism
Spine growth & development
Spine metabolism
Telencephalon cytology
Telencephalon metabolism
Telencephalon pathology
Tubulin metabolism
Zebrafish
Zebrafish Proteins genetics
Zebrafish Proteins metabolism
Brain metabolism
Cilia metabolism
Ependyma metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 2211-1247
- Volume :
- 37
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Cell reports
- Publication Type :
- Academic Journal
- Accession number :
- 34610312
- Full Text :
- https://doi.org/10.1016/j.celrep.2021.109775