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2D MOF-Based Photoelectrochemical Aptasensor for SARS-CoV-2 Spike Glycoprotein Detection.
- Source :
-
ACS applied materials & interfaces [ACS Appl Mater Interfaces] 2021 Oct 27; Vol. 13 (42), pp. 49754-49761. Date of Electronic Publication: 2021 Oct 17. - Publication Year :
- 2021
-
Abstract
- A reliable and sensitive detection approach for SARS-CoV 2 is essential for timely infection diagnosis and transmission prevention. Here, a two-dimensional (2D) metal-organic framework (MOF)-based photoelectrochemical (PEC) aptasensor with high sensitivity and stability for SARS-CoV 2 spike glycoprotein (S protein) detection was developed. The PEC aptasensor was constructed by a plasmon-enhanced photoactive material (namely, Au NPs/Yb-TCPP) with a specific DNA aptamer against S protein. The Au NPs/Yb-TCPP fabricated by in situ growth of Au NPs on the surface of 2D Yb-TCPP nanosheets showed a high electron-hole (e-h) separation efficiency due to the enhancement effect of plasmon, resulting in excellent photoelectric performance. The modified DNA aptamer on the surface of Au NPs/Yb-TCPP can bind with S protein with high selectivity, thus decreasing the photocurrent of the system due to the high steric hindrance and low conductivity of the S protein. The established PEC aptasensor demonstrated a highly sensitive detection for S protein with a linear response range of 0.5-8 μg/mL with a detection limit of 72 ng/mL. This work presented a promising way for the detection of SARS-CoV 2, which may conduce to the impetus of clinic diagnostics.
- Subjects :
- Base Sequence
Biosensing Techniques instrumentation
COVID-19 diagnosis
DNA chemistry
Electrochemical Techniques instrumentation
Electrochemical Techniques methods
Electrodes
Gold chemistry
Gold radiation effects
Humans
Immobilized Nucleic Acids chemistry
Light
Limit of Detection
Metal Nanoparticles chemistry
Metal Nanoparticles radiation effects
Pharynx virology
Photochemical Processes
Porphyrins chemistry
Spike Glycoprotein, Coronavirus chemistry
Ytterbium chemistry
Aptamers, Nucleotide chemistry
Biosensing Techniques methods
Metal-Organic Frameworks chemistry
SARS-CoV-2 chemistry
Spike Glycoprotein, Coronavirus analysis
Subjects
Details
- Language :
- English
- ISSN :
- 1944-8252
- Volume :
- 13
- Issue :
- 42
- Database :
- MEDLINE
- Journal :
- ACS applied materials & interfaces
- Publication Type :
- Academic Journal
- Accession number :
- 34657424
- Full Text :
- https://doi.org/10.1021/acsami.1c17574