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Establishment and characterization of NCC-UPS3-C1: a novel patient-derived cell line of undifferentiated pleomorphic sarcoma.
- Source :
-
Human cell [Hum Cell] 2022 Jan; Vol. 35 (1), pp. 384-391. Date of Electronic Publication: 2021 Oct 19. - Publication Year :
- 2022
-
Abstract
- Undifferentiated pleomorphic sarcoma (UPS), previously termed malignant fibrous histiocytoma, is one of the most aggressive sarcomas with no identifiable line of differentiation. Although the molecular mechanism of oncogenesis in UPS has not been clarified, radiation exposure is considered to be a risk factor in the development of UPS. In the treatment of UPS, surgical treatment remains the most important modality. While chemotherapy is considered in unresectable or metastatic cases, UPS is known to be refractory to conventional chemotherapy, leading to an unfavorable prognosis. To improve the clinical outcome of this condition, novel treatment methods are urgently needed. Patient-derived cell lines are essential tools in preclinical studies. However, owing to the rarity of UPS, only four UPS cell lines are publicly available. Thus, we established a novel UPS cell line, NCC-UPS3-C1, using a surgically resected tumor from a patient with radiation-associated UPS. NCC-UPS3-C1 cells had multiple genomic deletions including the tumor suppressor genes CDKN2A and CDKN2B. NCC-UPS3-C1 cells demonstrated constant growth, spheroid formation, and aggressive invasion ability. We also conducted a screening test using 214 drugs and identified that the histone deacetylase inhibitor, romidepsin, is highly effective on NCC-UPS3-C1 cells. Thus, we concluded that the NCC-UPS3-C1 cell line is a useful tool in preclinical studies for UPS.<br /> (© 2021. Japan Human Cell Society.)
- Subjects :
- Antineoplastic Agents pharmacology
Cell Line, Tumor
Cyclin-Dependent Kinase Inhibitor p15 genetics
Cyclin-Dependent Kinase Inhibitor p16 genetics
Depsipeptides pharmacology
Drug Screening Assays, Antitumor
Gene Deletion
Histiocytoma, Malignant Fibrous genetics
Histone Deacetylase Inhibitors pharmacology
Humans
Soft Tissue Neoplasms genetics
Histiocytoma, Malignant Fibrous drug therapy
Histiocytoma, Malignant Fibrous pathology
Soft Tissue Neoplasms drug therapy
Soft Tissue Neoplasms pathology
Subjects
Details
- Language :
- English
- ISSN :
- 1749-0774
- Volume :
- 35
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Human cell
- Publication Type :
- Academic Journal
- Accession number :
- 34665443
- Full Text :
- https://doi.org/10.1007/s13577-021-00633-w