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Molecular insights into receptor binding of recent emerging SARS-CoV-2 variants.
- Source :
-
Nature communications [Nat Commun] 2021 Oct 20; Vol. 12 (1), pp. 6103. Date of Electronic Publication: 2021 Oct 20. - Publication Year :
- 2021
-
Abstract
- Multiple SARS-CoV-2 variants of concern (VOCs) have been emerging and some have been linked to an increase in case numbers globally. However, there is yet a lack of understanding of the molecular basis for the interactions between the human ACE2 (hACE2) receptor and these VOCs. Here we examined several VOCs including Alpha, Beta, and Gamma, and demonstrate that five variants receptor-binding domain (RBD) increased binding affinity for hACE2, and four variants pseudoviruses increased entry into susceptible cells. Crystal structures of hACE2-RBD complexes help identify the key residues facilitating changes in hACE2 binding affinity. Additionally, soluble hACE2 protein efficiently prevent most of the variants pseudoviruses. Our findings provide important molecular information and may help the development of novel therapeutic and prophylactic agents targeting these emerging mutants.<br /> (© 2021. The Author(s).)
- Subjects :
- Amino Acid Sequence
Angiotensin-Converting Enzyme 2 genetics
Angiotensin-Converting Enzyme 2 isolation & purification
Angiotensin-Converting Enzyme 2 ultrastructure
Animals
Cell Line, Tumor
Crystallography, X-Ray
HEK293 Cells
Humans
Molecular Dynamics Simulation
Mutation
Recombinant Proteins genetics
Recombinant Proteins isolation & purification
Recombinant Proteins metabolism
Recombinant Proteins ultrastructure
SARS-CoV-2 genetics
Sf9 Cells
Spike Glycoprotein, Coronavirus genetics
Spike Glycoprotein, Coronavirus isolation & purification
Spike Glycoprotein, Coronavirus ultrastructure
Spodoptera
Surface Plasmon Resonance
Virus Attachment
Virus Internalization
Angiotensin-Converting Enzyme 2 metabolism
COVID-19 virology
Protein Interaction Domains and Motifs genetics
SARS-CoV-2 metabolism
Spike Glycoprotein, Coronavirus metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 2041-1723
- Volume :
- 12
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Nature communications
- Publication Type :
- Academic Journal
- Accession number :
- 34671049
- Full Text :
- https://doi.org/10.1038/s41467-021-26401-w