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Single-domain antibody screening by is PLA-seq.
- Source :
-
Life science alliance [Life Sci Alliance] 2021 Oct 21; Vol. 5 (1). Date of Electronic Publication: 2021 Oct 21 (Print Publication: 2022). - Publication Year :
- 2021
-
Abstract
- Single-domain antibody (sdAb) holds the promising strategies for diverse research and translational applications. Here, we describe a method for the adaptation of the in situ proximity ligation assay ( is PLA) followed by sequencing ( is PLA-seq) to facilitate screening of a high-sensitive, high-throughput sdAb library for a given protein at subcellular and single-cell resolution. Based on the sequence of complementarity-determining region 3 (CDR3), the recombinant sdAb can be produced for in vitro and in vivo utilities. This method provides a general means to identify the functional measure of sdAb and its complementary epitopes and its potential applications to investigate cellular processes.<br /> (© 2021 Yin et al.)
- Subjects :
- Base Sequence
Cell Line
Complementarity Determining Regions chemistry
Complementarity Determining Regions immunology
Gene Library
Humans
Immunophenotyping
Molecular Imaging
Sequestosome-1 Protein immunology
Single-Domain Antibodies chemistry
High-Throughput Screening Assays methods
Single-Domain Antibodies immunology
Subjects
Details
- Language :
- English
- ISSN :
- 2575-1077
- Volume :
- 5
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Life science alliance
- Publication Type :
- Academic Journal
- Accession number :
- 34675071
- Full Text :
- https://doi.org/10.26508/lsa.202101115