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Assessment of Anti-Inflammatory and Antioxidant Effects of Citrus unshiu Peel (CUP) Flavonoids on LPS-Stimulated RAW 264.7 Cells.

Authors :
Karthikeyan A
Kim HH
Preethi V
Moniruzzaman M
Lee KH
Kalaiselvi S
Kim GS
Min T
Source :
Plants (Basel, Switzerland) [Plants (Basel)] 2021 Oct 18; Vol. 10 (10). Date of Electronic Publication: 2021 Oct 18.
Publication Year :
2021

Abstract

Citrus unshiu is a popular medicinal herb in several Asian countries, in particular South Korea. C. unshiu peel (CUP) has several biologically active compounds, including flavonoids. Hence, this research aimed to label the flavonoids from CUP by HPLC-MS/MS analysis and examine their anti-inflammatory and antioxidant potential on LPS-stimulated RAW 264.7 macrophages. A total of four flavonoids (Rutin, naringin, hesperidin, and poncirin) were characterized, and their contents were quantified from CUP. It showed that the naringin is rich in CUP. Further, treatment with the flavonoids at concentrations of 2.5 and 5 μg/mL had no effect on the cell viability of RAW 264.7 macrophages. On the other hand, it decreased the production and expression of inflammatory mediators and pro-inflammatory cytokines such as NO, PGE <subscript>2</subscript> , TNF-α, IL-1β, iNOS, and COX2 in the LPS-stimulated RAW 264.7 macrophages. In addition, flavonoids treatment inhibited the NF-κB activation by downregulating the p-p65 and p-IκBα proteins expression. Furthermore, reactive oxygen species (ROS) production considerably decreased at the same concentrations while antioxidant enzyme activity increased in the LPS-stimulated RAW 264.7 macrophages. Collectively, our results show that CUP flavonoids have the potential to decrease inflammation and oxidative damage.

Details

Language :
English
ISSN :
2223-7747
Volume :
10
Issue :
10
Database :
MEDLINE
Journal :
Plants (Basel, Switzerland)
Publication Type :
Academic Journal
Accession number :
34686018
Full Text :
https://doi.org/10.3390/plants10102209