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High-Dimensional Immunophenotyping with 37-Color Panel Using Full-Spectrum Cytometry.
- Source :
-
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2022; Vol. 2386, pp. 43-60. - Publication Year :
- 2022
-
Abstract
- A comprehensive study of the cellular components of the immune system demands both deep and broad immunophenotyping of numerous cell subsets in an effective and practical way. Novel full-spectrum technology reveals the complete emission spectrum of each dye maximizing the amount of information that can be obtained on a single sample regarding conventional flow cytometry and provide an expanded knowledge of biological processes. In this chapter, we describe a 37-color protocol that allows to identify more than 45 different cell populations on whole blood samples of SARS-CoV-2-infected patients.<br /> (© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
- Subjects :
- Color
Humans
Immune System
COVID-19 blood
Flow Cytometry
Immunophenotyping methods
Subjects
Details
- Language :
- English
- ISSN :
- 1940-6029
- Volume :
- 2386
- Database :
- MEDLINE
- Journal :
- Methods in molecular biology (Clifton, N.J.)
- Publication Type :
- Academic Journal
- Accession number :
- 34766264
- Full Text :
- https://doi.org/10.1007/978-1-0716-1771-7_4