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Establishment and characterization of NCC-MPNST6-C1: a novel patient-derived cell line of malignant peripheral nerve sheath tumors.
- Source :
-
Human cell [Hum Cell] 2022 Jan; Vol. 35 (1), pp. 400-407. Date of Electronic Publication: 2021 Nov 14. - Publication Year :
- 2022
-
Abstract
- Malignant peripheral nerve sheath tumors (MPNSTs) are a rare subtype of mesenchymal tumors that arise from the sheath of peripheral nerves. MPNSTs exhibit strong metastatic potential, leading to poor clinical outcomes. The clinical utility of radiation therapy and chemotherapy is marginal with respect to overall survival and effective systematic therapies for MPNSTs are still needed to improve patient outcome. Although patient-derived cell lines are an essential tool for the development of novel therapies, only a limited number of cell lines have been reported and are available from cell banks. Thus, we established the novel MPNST cell line, NCC-MPNST6-C1, using surgically resected MPNST tissue. The NCC-MPNST6-C1 cells retained copy-number alterations similar to those of the original tumors and demonstrated constant proliferation, spheroid formation, and invasion capability in vitro, which reflected the malignant features of the original tumor tissue. While the NCC-MPNST6-C1 cells did not exhibit tumorigenesis in nude mice, their use in drug screening resulted in anti-cancer agents with low IC <subscript>50</subscript> values. Hence, we conclude that the NCC-MPNST6-C1 cell line is a useful resource for the study of MPNSTs.<br /> (© 2021. The Author(s) under exclusive licence to Japan Human Cell Society.)
- Subjects :
- Animals
Antineoplastic Agents pharmacology
Cell Line, Tumor
Cell Proliferation drug effects
Dose-Response Relationship, Drug
Drug Screening Assays, Antitumor
Humans
Mice, Nude
Neoplasm Invasiveness
Spheroids, Cellular pathology
Mice
Nerve Sheath Neoplasms pathology
Peripheral Nervous System Neoplasms pathology
Subjects
Details
- Language :
- English
- ISSN :
- 1749-0774
- Volume :
- 35
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Human cell
- Publication Type :
- Academic Journal
- Accession number :
- 34775549
- Full Text :
- https://doi.org/10.1007/s13577-021-00643-8