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Spatial metabolomics using imaging mass spectrometry to identify the localization of asparaptine A in Asparagus officinalis .
- Source :
-
Plant biotechnology (Tokyo, Japan) [Plant Biotechnol (Tokyo)] 2021 Sep 25; Vol. 38 (3), pp. 311-315. - Publication Year :
- 2021
-
Abstract
- Spatial metabolomics uses imaging mass spectrometry (IMS) to localize metabolites within tissue section. Here, we performed matrix-assisted laser desorption/ionization-Fourier transform ion cyclotron resonance-IMS (MALDI-FTICR-IMS) to identify the localization of asparaptine A, a naturally occurring inhibitor of angiotensin-converting enzyme, in green spears of asparagus ( Asparagus officinalis ). Spatial metabolome data were acquired in an untargeted manner. Segmentation analysis using the data characterized tissue-type-dependent and independent distribution patterns in cross-sections of asparagus spears. Moreover, asparaptine A accumulated at high levels in developing lateral shoot tissues. Quantification of asparaptine A in lateral shoots using liquid chromatography-tandem mass spectrometry (LC-MS/MS) validated the IMS analysis. These results provide valuable information for understanding the function of asparaptine A in asparagus, and identify the lateral shoot as a potential region of interest for multiomics studies to examine gene-to-metabolite associations in the asparaptine A biosynthesis.<br /> (© 2021 Japanese Society for Plant Biotechnology.)
Details
- Language :
- English
- ISSN :
- 1342-4580
- Volume :
- 38
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Plant biotechnology (Tokyo, Japan)
- Publication Type :
- Academic Journal
- Accession number :
- 34782817
- Full Text :
- https://doi.org/10.5511/plantbiotechnology.21.0504b