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Prolonged storage of purified granulocyte concentrates: Introduction of a new purification method.
- Source :
-
Transfusion [Transfusion] 2022 Jan; Vol. 62 (1), pp. 194-204. Date of Electronic Publication: 2021 Nov 16. - Publication Year :
- 2022
-
Abstract
- Background: Use of donor granulocyte concentrate (GC) has been limited due to its short storage time of 6-24 h, which is partially due to residual red blood cells (RBCs) and platelets and the resulting lactate production leading to an acidotic milieu. To increase this storage time, we developed a closed system procedure compatible with standard blood bank technologies to remove RBC and platelets and to enrich the GC.<br />Methods: Standard GCs (sGCs) were sedimented, washed twice with 0.9% sodium chloride (NaCl), and resuspended in blood group-identical fresh frozen plasma. The resulting purified GCs (pGCs) were then stored in platelet bags at a cell concentration of about 5 × 10 <superscript>7</superscript>  ± 1.8 × 10 <superscript>7</superscript> leukocytes/ml without agitation at room temperature for up to 72 h. Cell count and viability, pH, blood gases, phagocytosis, and oxidative burst were monitored daily.<br />Results: A significant reduction in RBC (98%) through sedimentation, and platelets (96%) by washing, purified the white blood cell (WBC) population and enriched the granulocytes to 96% of the WBC in the pGC. After 72 h of storage, over 90% of the initial WBC count of pGC remained, was viable (≥97%), and the granulocytes exhibited a high phagocytosis and oxidative burst functionality, comparable to sGC after 24 h.<br />Conclusion: Purification extends the maximum storage period of GC from 24 to 72 h and may therefore improve the availability of GC and its clinical use.<br /> (© 2021 The Authors. Transfusion published by Wiley Periodicals LLC on behalf of AABB.)
Details
- Language :
- English
- ISSN :
- 1537-2995
- Volume :
- 62
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Transfusion
- Publication Type :
- Academic Journal
- Accession number :
- 34783358
- Full Text :
- https://doi.org/10.1111/trf.16732