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Ricin B lectin-like proteins of the microsporidian Encephalitozoon cuniculi and Anncaliia algerae are involved in host-cell invasion.
- Source :
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Parasitology international [Parasitol Int] 2022 Apr; Vol. 87, pp. 102518. Date of Electronic Publication: 2021 Nov 20. - Publication Year :
- 2022
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Abstract
- Microsporidia are obligate intracellular pathogens capable of infecting a wide variety of hosts ranging from invertebrates to vertebrates. The infection process requires a step of prior adherence of Microsporidia to the surface of host cells. A few studies demonstrated the involvement of proteins containing a ricin-B lectin (RBL) domain in parasite infection. In this study Anncalia algerae and Encephalitozoon cuniculi genomes were screened by bioinformatic analysis to identify proteins with an extracellular prediction and possessing RBL-type carbohydrate-binding domains, being both potentially relevant factors contributing to host cell adherence. Three proteins named AaRBLL-1 and AaRBLL-2 from A. algerae and EcRBLL-1 from E. cuniculi, were selected and comparative analysis of sequences suggested their belonging to a multigenic family, with a conserved structural RBL domain despite a significant amino acid sequence divergence. The production of recombinant proteins and antibodies against the three proteins allowed their subcellular localization on the spore wall and/or the polar tube. Adherence inhibition assays based on pre-treatments with recombinant proteins or antibodies highlighted the significant decrease of the proliferation of both E. cuniculi and A. algerae, strongly suggesting that these proteins are involved in the infection process.<br /> (Copyright © 2021 Elsevier B.V. All rights reserved.)
- Subjects :
- Animals
Cell Line
Computational Biology
Dogs
Encephalitozoon cuniculi genetics
Encephalitozoon cuniculi immunology
Humans
Madin Darby Canine Kidney Cells
Microsporidia genetics
Microsporidia immunology
Rabbits
Recombinant Proteins genetics
Spores, Fungal immunology
Spores, Fungal isolation & purification
Encephalitozoon cuniculi chemistry
Fungal Proteins physiology
Microsporidia chemistry
Ricin metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1873-0329
- Volume :
- 87
- Database :
- MEDLINE
- Journal :
- Parasitology international
- Publication Type :
- Academic Journal
- Accession number :
- 34808329
- Full Text :
- https://doi.org/10.1016/j.parint.2021.102518