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In situ-formed adhesive hyaluronic acid hydrogel with prolonged amnion-derived conditioned medium release for diabetic wound repair.

Authors :
Zhang Y
Zheng Y
Shu F
Zhou R
Bao B
Xiao S
Li K
Lin Q
Zhu L
Xia Z
Source :
Carbohydrate polymers [Carbohydr Polym] 2022 Jan 15; Vol. 276, pp. 118752. Date of Electronic Publication: 2021 Oct 16.
Publication Year :
2022

Abstract

Hydrogels have long been used for encapsulating stem cell-derived conditioned mediums to achieve skin regeneration after wounding. However, inappropriate mechanical strength, low adhesion and low elasticity limit their clinical application. To address these challenges, we engineered a hyaluronic acid-based hydrogel grafted with methacrylic anhydride and N-(2-aminoethyl)-4-[4-(hydroxymethyl)-2-methoxy-5-nitrophenoxy]-butanamide (NB) groups to encapsulate a lyophilized amnion-derived conditioned medium (AM-CM). This hydrogel can photopolymerize in situ within 3 s by photo-initiated free-radical crosslinking between methacrylate moieties. Meanwhile, the formed o-nitrosobenzaldehyde groups by photo-irradiation could covalently bond with the amino groups of tissue surface, which allowed strong tissue adhesion. Furthermore, the hydrogel possessed excellent mechanical properties, high elasticity, favorable biocompatibility and prolonged AM-CM release. Our further vitro and in vivo studies showed that the hydrogel significantly accelerated diabetic wound healing by regulating macrophage polarization and promoting angiogenesis. The engineered hydrogel with AM-CM release has high potential to treat chronic wounds in clinics.<br /> (Copyright © 2021 The Authors. Published by Elsevier Ltd.. All rights reserved.)

Details

Language :
English
ISSN :
1879-1344
Volume :
276
Database :
MEDLINE
Journal :
Carbohydrate polymers
Publication Type :
Academic Journal
Accession number :
34823781
Full Text :
https://doi.org/10.1016/j.carbpol.2021.118752