Back to Search
Start Over
In situ-formed adhesive hyaluronic acid hydrogel with prolonged amnion-derived conditioned medium release for diabetic wound repair.
- Source :
-
Carbohydrate polymers [Carbohydr Polym] 2022 Jan 15; Vol. 276, pp. 118752. Date of Electronic Publication: 2021 Oct 16. - Publication Year :
- 2022
-
Abstract
- Hydrogels have long been used for encapsulating stem cell-derived conditioned mediums to achieve skin regeneration after wounding. However, inappropriate mechanical strength, low adhesion and low elasticity limit their clinical application. To address these challenges, we engineered a hyaluronic acid-based hydrogel grafted with methacrylic anhydride and N-(2-aminoethyl)-4-[4-(hydroxymethyl)-2-methoxy-5-nitrophenoxy]-butanamide (NB) groups to encapsulate a lyophilized amnion-derived conditioned medium (AM-CM). This hydrogel can photopolymerize in situ within 3 s by photo-initiated free-radical crosslinking between methacrylate moieties. Meanwhile, the formed o-nitrosobenzaldehyde groups by photo-irradiation could covalently bond with the amino groups of tissue surface, which allowed strong tissue adhesion. Furthermore, the hydrogel possessed excellent mechanical properties, high elasticity, favorable biocompatibility and prolonged AM-CM release. Our further vitro and in vivo studies showed that the hydrogel significantly accelerated diabetic wound healing by regulating macrophage polarization and promoting angiogenesis. The engineered hydrogel with AM-CM release has high potential to treat chronic wounds in clinics.<br /> (Copyright © 2021 The Authors. Published by Elsevier Ltd.. All rights reserved.)
- Subjects :
- Adhesives
Anhydrides chemistry
Animals
Cell Proliferation drug effects
Culture Media, Conditioned
Diabetes Mellitus metabolism
Human Umbilical Vein Endothelial Cells
Humans
Hyaluronic Acid chemistry
Hydrogels chemistry
Macrophages drug effects
Male
Mice
Mice, Inbred C57BL
Rheology methods
Skin drug effects
Stem Cells metabolism
Tissue Adhesions metabolism
Amnion chemistry
Diabetes Mellitus drug therapy
Hyaluronic Acid pharmacology
Hydrogels pharmacology
Wound Healing drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1879-1344
- Volume :
- 276
- Database :
- MEDLINE
- Journal :
- Carbohydrate polymers
- Publication Type :
- Academic Journal
- Accession number :
- 34823781
- Full Text :
- https://doi.org/10.1016/j.carbpol.2021.118752