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De novo developed protein binders mimicking Interferon lambda signaling.
- Source :
-
The FEBS journal [FEBS J] 2022 May; Vol. 289 (9), pp. 2672-2684. Date of Electronic Publication: 2021 Dec 07. - Publication Year :
- 2022
-
Abstract
- We hereby describe the process of design and selection of nonantibody protein binders mimicking cytokine signaling. We chose to mimic signaling of IFN-λ1, type 3 interferon (also known as IL-29) for its novelty and the importance of its biological functions. All four known interferons λ signal through binding to the extracellular domains of IL-28 receptor 1 (IL-28R1) and IL-10 receptor 2 (IL-10R2). Our binders were therefore trained to bind both receptors simultaneously. The bifunctional binder molecules were developed by yeast display, a method of directed evolution. The signaling capacity of the bivalent binders was tested by measuring phosphorylation of the JAK/STAT signaling pathway and production of mRNA of six selected genes naturally induced by IFN- λ1 in human cell lines. The newly developed bivalent binders offer opportunities to study cytokine-related biological functions and modulation of the cell behavior by receptor activation on the cell surfaces alternative to the use of natural IFN-λ.<br /> (© 2021 Federation of European Biochemical Societies.)
Details
- Language :
- English
- ISSN :
- 1742-4658
- Volume :
- 289
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- The FEBS journal
- Publication Type :
- Academic Journal
- Accession number :
- 34826176
- Full Text :
- https://doi.org/10.1111/febs.16300