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Effects of Metallic and Carbon-Based Nanomaterials on Human Pancreatic Cancer Cell Lines AsPC-1 and BxPC-3.
- Source :
-
International journal of molecular sciences [Int J Mol Sci] 2021 Nov 09; Vol. 22 (22). Date of Electronic Publication: 2021 Nov 09. - Publication Year :
- 2021
-
Abstract
- Pancreatic cancer, due to its asymptomatic development and drug-resistance, is difficult to cure. As many metallic and carbon-based nanomaterials have shown anticancer properties, we decided to investigate their potential use as anticancer agents against human pancreatic adenocarcinoma. The objective of the study was to evaluate the toxic properties of the following nanomaterials: silver (Ag), gold (Au), platinum (Pt), graphene oxide (GO), diamond (ND), and fullerenol (C <subscript>60</subscript> (OH) <subscript>40</subscript> ) against the cell lines BxPC-3, AsPC-1, HFFF-2, and HS-5. The potential cytotoxic properties were evaluated by the assessment of the cell morphology, cell viability, and cell membrane damage. The cancer cell responses to GO and ND were analysed by determination of changes in the levels of 40 different pro-inflammatory proteins. Our studies revealed that the highest cytotoxicity was obtained after the ND treatment. Moreover, BxPC-3 cells were more sensitive to ND than AsPC-1 cells due to the ND-induced ROS production. Furthermore, in both of the cancer cell lines, ND caused an increased level of IL-8 and a decreased level of TIMP-2, whereas GO caused only decreased levels of TIMP-2 and ICAM-1 proteins. This work provides important data on the toxicity of various nanoparticles against pancreatic adenocarcinoma cell lines.
- Subjects :
- Antineoplastic Agents chemistry
Apoptosis drug effects
Cell Line, Tumor
Cell Survival drug effects
Diamond chemistry
Diamond pharmacology
Fullerenes chemistry
Fullerenes pharmacology
Gene Expression Regulation, Neoplastic drug effects
Gold chemistry
Gold pharmacology
Graphite chemistry
Graphite pharmacology
Humans
Nanostructures therapeutic use
Pancreatic Neoplasms genetics
Pancreatic Neoplasms pathology
Platinum chemistry
Platinum pharmacology
Silver chemistry
Silver pharmacology
Antineoplastic Agents pharmacology
Nanostructures chemistry
Neoplasm Proteins genetics
Pancreatic Neoplasms drug therapy
Subjects
Details
- Language :
- English
- ISSN :
- 1422-0067
- Volume :
- 22
- Issue :
- 22
- Database :
- MEDLINE
- Journal :
- International journal of molecular sciences
- Publication Type :
- Academic Journal
- Accession number :
- 34829982
- Full Text :
- https://doi.org/10.3390/ijms222212100