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HATMSC Secreted Factors in the Hydrogel as a Potential Treatment for Chronic Wounds-In Vitro Study.
- Source :
-
International journal of molecular sciences [Int J Mol Sci] 2021 Nov 12; Vol. 22 (22). Date of Electronic Publication: 2021 Nov 12. - Publication Year :
- 2021
-
Abstract
- Mesenchymal stem cells (MSCs) can improve chronic wound healing; however, recent studies suggest that the therapeutic effect of MSCs is mediated mainly through the growth factors and cytokines secreted by these cells, referred to as the MSC secretome. To overcome difficulties related to the translation of cell therapy into clinical use such as efficacy, safety and cost, we propose a hydrogel loaded with a secretome from the recently established human adipose tissue mesenchymal stem cell line (HATMSC2) as a potential treatment for chronic wounds. Biocompatibility and biological activity of hydrogel-released HATMSC2 supernatant were investigated in vitro by assessing the proliferation and metabolic activity of human fibroblast, endothelial cells and keratinocytes. Hydrogel degradation was measured using hydroxyproline assay while protein released from the hydrogel was assessed by interleukin-8 (IL-8) and macrophage chemoattractant protein-1 (MCP-1) ELISAs. Pro-angiogenic activity of the developed treatment was assessed by tube formation assay while the presence of pro-angiogenic miRNAs in the HATMSC2 supernatant was investigated using real-time RT-PCR. The results demonstrated that the therapeutic effect of the HATMSC2-produced factors is maintained following incorporation into collagen hydrogel as confirmed by increased proliferation of skin-origin cells and improved angiogenic properties of endothelial cells. In addition, HATMSC2 supernatant revealed antimicrobial activity, and which therefore, in combination with the hydrogel has a potential to be used as advanced wound-healing dressing.
- Subjects :
- Anti-Infective Agents chemistry
Anti-Infective Agents pharmacology
Bacteria classification
Bacteria drug effects
Bacteria growth & development
Cell Line
Cell Proliferation drug effects
Cell Proliferation genetics
Cell Survival drug effects
Cell Survival genetics
Culture Media, Conditioned chemistry
Endothelial Cells drug effects
Endothelial Cells metabolism
Endothelial Cells microbiology
Fibroblasts drug effects
Fibroblasts metabolism
Fibroblasts microbiology
Humans
Hydrogels chemistry
Keratinocytes drug effects
Keratinocytes metabolism
Keratinocytes microbiology
Mesenchymal Stem Cells cytology
MicroRNAs genetics
Skin cytology
Skin microbiology
Adipose Tissue cytology
Culture Media, Conditioned pharmacology
Hydrogels pharmacology
Mesenchymal Stem Cells metabolism
Secretome metabolism
Skin metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1422-0067
- Volume :
- 22
- Issue :
- 22
- Database :
- MEDLINE
- Journal :
- International journal of molecular sciences
- Publication Type :
- Academic Journal
- Accession number :
- 34830121
- Full Text :
- https://doi.org/10.3390/ijms222212241