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Biochanin A as an α-hemolysin inhibitor for combating methicillin-resistant Staphylococcus aureus infection.
- Source :
-
World journal of microbiology & biotechnology [World J Microbiol Biotechnol] 2021 Nov 27; Vol. 38 (1), pp. 6. Date of Electronic Publication: 2021 Nov 27. - Publication Year :
- 2021
-
Abstract
- Methicillin-resistant Staphylococcus aureus (MRSA) is a multidrug-resistant pathogen that poses a significant risk to global health today. In S. aureus, α-hemolysin is an important virulence factor as it contributes to the capacity of the bacteria to infect the host. Here, we showed that biochanin A (bioA), an isoflavone present in red clover, cabbage and alfalfa, effectively inhibited hemolytic activity at a dose as low as 32 μg/mL. Further, western blot and RT-qPCR data showed that bioA reduced the production and expression of MRSA hemolysin in a dose-dependent manner. In addition, when different concentrations of bioA were added to a coculture system of A549 cells and S. aureus, it could significantly decrease cell injury. Importantly, the in vivo study showed that bioA could protect mice from pneumonia caused by a lethal dose of MRSA, as evidenced by improving their survival and reducing the number of bacterial colonies in lung tissues, the secretion of hemolysin into alveolar lavage fluid and the degree of pulmonary edema. In conclusion, biochanin A protected the host from MRSA infection by inhibiting the expression of the hemolysin of MRSA, which may provide experimental evidence for its development to a potential anti-MRSA drug.<br /> (© 2021. The Author(s), under exclusive licence to Springer Nature B.V.)
- Subjects :
- A549 Cells
Animals
Anti-Bacterial Agents pharmacology
Bacterial Proteins genetics
Bacterial Proteins metabolism
Disease Models, Animal
Dose-Response Relationship, Drug
Female
Gene Expression Regulation, Bacterial drug effects
Genistein pharmacology
Hemolysin Proteins genetics
Hemolysis drug effects
Humans
Methicillin-Resistant Staphylococcus aureus genetics
Methicillin-Resistant Staphylococcus aureus pathogenicity
Mice
Pneumonia microbiology
Staphylococcal Infections microbiology
Anti-Bacterial Agents administration & dosage
Genistein administration & dosage
Hemolysin Proteins metabolism
Methicillin-Resistant Staphylococcus aureus drug effects
Pneumonia drug therapy
Staphylococcal Infections drug therapy
Subjects
Details
- Language :
- English
- ISSN :
- 1573-0972
- Volume :
- 38
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- World journal of microbiology & biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 34837116
- Full Text :
- https://doi.org/10.1007/s11274-021-03182-4