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Detection of Viroids by RT-PCR.
- Source :
-
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2022; Vol. 2316, pp. 143-151. - Publication Year :
- 2022
-
Abstract
- Reverse transcription-polymerase chain reaction (RT-PCR) is an effective method for detecting the presence of viroids in plant tissue. Viroid RNA is converted to cDNA and amplified to detectable levels, making it a fast and useful detection tool, even when the viroid is present at low levels. Methods of viroid detection using conventional RT-PCR are described in this chapter.<br /> (© 2022. Springer Science+Business Media, LLC, part of Springer Nature.)
Details
- Language :
- English
- ISSN :
- 1940-6029
- Volume :
- 2316
- Database :
- MEDLINE
- Journal :
- Methods in molecular biology (Clifton, N.J.)
- Publication Type :
- Academic Journal
- Accession number :
- 34845692
- Full Text :
- https://doi.org/10.1007/978-1-0716-1464-8_13