Back to Search
Start Over
Carbon dots and gold nanoclusters assisted construction of a ratiometric fluorescent biosensor for detection of Gram-negative bacteria.
- Source :
-
Food chemistry [Food Chem] 2022 Apr 16; Vol. 374, pp. 131750. Date of Electronic Publication: 2021 Dec 01. - Publication Year :
- 2022
-
Abstract
- A core-satellite nanocomposite was prepared by encapsulating the photostable blue carbon dots (BCDs) in the core of silica as the reference signal readout, and the target-sensitive gold nanoclusters (AuNCs) covalently linked to the surface of silica as the respond signal readout. The nanocomposite (BCD@SiO <subscript>2</subscript> @AuNC) was used as a ratiometric fluorescent sensor to realize the selective detection of Gram-negative bacteria. The detection principle was based on the quenching of Cu <superscript>2+</superscript> toward AuNCs and the reduction of Gram-negative bacteria toward Cu <superscript>2+</superscript> . The sensor exhibited good selectivity toward Gram-negative bacteria owing to the copper-homeostasis mechanism possessed by the bacteria. The sensor demonstrated linear response to the logarithm concentration of Gram-negative bacteria with determination coefficients higher than 0.912. The feasibility of the sensor was verified by analysis of Gram-negative bacteria in eggshell, swimming pool water, as well as Chinese cabbage samples with recoveries ranging from 93.9% to 109%.<br /> (Copyright © 2021 Elsevier Ltd. All rights reserved.)
Details
- Language :
- English
- ISSN :
- 1873-7072
- Volume :
- 374
- Database :
- MEDLINE
- Journal :
- Food chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 34871851
- Full Text :
- https://doi.org/10.1016/j.foodchem.2021.131750