The effect of chemicals on the activity of deglycosylated Aureobasidiumβ-fructofuranosidases.

The effects of chemicals such as metal ions and typical organic enzyme inhibitors on the activity of deglycosylated β-fructofuranosidases (P-1 and P-2) from Aureobasidium were observed and compared with those of native enzymes. P-1 and P-2 enzymes became sensitive to metal ions, such as Fe ²⁺ , Co ²⁺ , Ni ²⁺ and Al ³⁺ , after deglycosylation. The enzymes were also inhibited by a sulphydryl reagent (monoiodoacetic acid), a peptide-hydrolysing reagent (hydroxylamine) and chelating reagents (sodium citrate, EDTA and sodium azide) after deglycosylation. The importance of deglycosylation for the determination of the true characteristics of the enzymes against chemicals is discussed.