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Glyoxal damages human aortic endothelial cells by perturbing the glutathione, mitochondrial membrane potential, and mitogen-activated protein kinase pathways.
- Source :
-
BMC cardiovascular disorders [BMC Cardiovasc Disord] 2021 Dec 18; Vol. 21 (1), pp. 603. Date of Electronic Publication: 2021 Dec 18. - Publication Year :
- 2021
-
Abstract
- Background: Exposure to glyoxal, the smallest dialdehyde, is associated with several diseases; humans are routinely exposed to glyoxal because of its ubiquitous presence in foods and the environment. The aim of this study was to examine the damage caused by glyoxal in human aortic endothelial cells.<br />Methods: Cell survival assays and quantitative fluorescence assays were performed to measure DNA damage; oxidative stress was detected by colorimetric assays and quantitative fluorescence, and the mitogen-activated protein kinase pathways were assessed using western blotting.<br />Results: Exposure to glyoxal was found to be linked to abnormal glutathione activity, the collapse of mitochondrial membrane potential, and the activation of mitogen-activated protein kinase pathways. However, DNA damage and thioredoxin oxidation were not induced by dialdehydes.<br />Conclusions: Intracellular glutathione, members of the mitogen-activated protein kinase pathways, and the mitochondrial membrane potential are all critical targets of glyoxal. These findings provide novel insights into the molecular mechanisms perturbed by glyoxal, and may facilitate the development of new therapeutics and diagnostic markers for cardiovascular diseases.<br /> (© 2021. The Author(s).)
- Subjects :
- Aorta enzymology
Aorta pathology
Cells, Cultured
DNA Damage
Endothelial Cells enzymology
Endothelial Cells pathology
Humans
Mitochondria enzymology
Mitochondria pathology
Oxidative Stress drug effects
Phosphorylation
Signal Transduction
Thioredoxins metabolism
Aorta drug effects
Endothelial Cells drug effects
Glutathione metabolism
Glyoxal toxicity
Membrane Potential, Mitochondrial drug effects
Mitochondria drug effects
Mitogen-Activated Protein Kinases metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1471-2261
- Volume :
- 21
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- BMC cardiovascular disorders
- Publication Type :
- Academic Journal
- Accession number :
- 34922451
- Full Text :
- https://doi.org/10.1186/s12872-021-02418-3