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Sedimentary Cobalt Protoporphyrin as a Potential Precursor of Prosthetic Heme Group for Bacteria Inhabiting Fossil Organic Matter-Rich Shale Rock.
- Source :
-
Biomolecules [Biomolecules] 2021 Dec 20; Vol. 11 (12). Date of Electronic Publication: 2021 Dec 20. - Publication Year :
- 2021
-
Abstract
- This study hypothesizes that bacteria inhabiting shale rock affect the content of the sedimentary cobalt protoporphyrin present in it and can use it as a precursor for heme synthesis. To verify this hypothesis, we conducted qualitative and quantitative comparative analyses of cobalt protoporphyrin as well as heme, and heme iron in shale rock that were (i) inhabited by bacteria in the field, (ii) treated with bacteria in the laboratory, and with (iii) bacterial culture on synthetic cobalt protoporphyrin. Additionally, we examined the above-mentioned samples for the presence of enzymes involved in the heme biosynthesis and uptake as well as hemoproteins. We found depletion of cobalt protoporphyrin and a much higher heme concentration in the shale rock inhabited by bacteria in the field as well as the shale rock treated with bacteria in the laboratory. Similarly, we observed the accumulation of protoporphyrin in bacterial cells grown on synthetic cobalt protoporphyrin. We detected numerous hemoproteins in metaproteome of bacteria inhabited shale rock in the field and in proteomes of bacteria inhabited shale rock and synthetic cobalt protoporhyrin in the laboratory, but none of them had all the enzymes involved in the heme biosynthesis. However, proteins responsible for heme uptake, ferrochelatase and sirohydrochlorin cobaltochelatase/sirohydrochlorin cobalt-lyase were detected in all studied samples.
- Subjects :
- Bacteria metabolism
Bacterial Proteins metabolism
Bacteriological Techniques
Culture Media chemistry
Ferrochelatase metabolism
Gene Expression Regulation, Bacterial
Geologic Sediments chemistry
Heme biosynthesis
Lyases metabolism
Proteomics
Protoporphyrins biosynthesis
Bacteria growth & development
Fossils microbiology
Geologic Sediments microbiology
Heme analysis
Protoporphyrins analysis
Subjects
Details
- Language :
- English
- ISSN :
- 2218-273X
- Volume :
- 11
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Biomolecules
- Publication Type :
- Academic Journal
- Accession number :
- 34944556
- Full Text :
- https://doi.org/10.3390/biom11121913