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An improved method for the purification of retinal S-antigen using selective hydrophobic adsorption chromatography.
- Source :
-
Journal of immunological methods [J Immunol Methods] 1987 Jun 26; Vol. 100 (1-2), pp. 147-52. - Publication Year :
- 1987
-
Abstract
- This paper describes the use of phenyl-Sepharose CL-4B as a solid-phase hydrophobic adsorbent in the purification of S-antigen from protein extracts of bovine, porcine and human retina. Chromatographic conditions were ascertained whereby the majority of contaminating proteins were bound to the adsorbent leaving S-antigen in the liquid phase. In combination with size fractionation on Ultrogel AcA, the method conveniently yielded porcine and bovine S-antigen preparations up to 100% purity. Immunogenicity of purified S-antigens was verified by induction of experimental autoimmune uveoretinitis in albino Lewis rats. The method is preparative in scale, fast in performance and yields S-antigen in high purity and antigenic potency.
Details
- Language :
- English
- ISSN :
- 0022-1759
- Volume :
- 100
- Issue :
- 1-2
- Database :
- MEDLINE
- Journal :
- Journal of immunological methods
- Publication Type :
- Academic Journal
- Accession number :
- 3496396
- Full Text :
- https://doi.org/10.1016/0022-1759(87)90183-9