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Fibronectin modified alginate coating enhances cell targeting and homing to bone marrow in BALB/c mice.

Authors :
Verma YK
Gurudutta GU
Source :
Journal of microencapsulation [J Microencapsul] 2022 Jan; Vol. 39 (1), pp. 49-60. Date of Electronic Publication: 2022 Jan 20.
Publication Year :
2022

Abstract

Stem cell homing to bone marrow (BM) suffers from premature differentiation of transfused cells within the blood stream, thereby reducing the efficiency of stem cell transplantation (SCT). This work is attempted to enhance the homing of cells in BM. Fibronectin modified alginate (A-F) was prepared and used to coat the cells. Biodistribution and survival advantage provided by A-F coating was evaluated in BALB/c mice. The A-F conjugate showed characteristic FT-IR peaks of alginate at 3308 cm <superscript>-1</superscript> and 1634 cm <superscript>-1</superscript> , and Fibronectin peak at 675 cm <superscript>-1</superscript> . The A-F coating prevented antibodies from binding to their respective cell surface receptors. The A-F coat abolished haemagglutination. Significant distribution of coated cells was observed in BM after 24 h. This provided protection to 7 Gy irradiated mice. The A-F coating showed no histological toxicity in vivo . The coating formulation is likely to be useful for shielding clinically relevant cell types to improve targeting for organ regeneration.

Details

Language :
English
ISSN :
1464-5246
Volume :
39
Issue :
1
Database :
MEDLINE
Journal :
Journal of microencapsulation
Publication Type :
Academic Journal
Accession number :
34985373
Full Text :
https://doi.org/10.1080/02652048.2022.2025934