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Influenza A Viruses Enter Host Cells via Extracellular Ca 2+ Influx-Involved Clathrin-Mediated Endocytosis.
- Source :
-
ACS applied bio materials [ACS Appl Bio Mater] 2021 Mar 15; Vol. 4 (3), pp. 2044-2051. Date of Electronic Publication: 2021 Jan 08. - Publication Year :
- 2021
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Abstract
- Influenza A virus (IAV) is internalized into its host cells by endocytosis, which involves many cellular proteins and molecules. In this study, we focus on the function of calcium ion (Ca <superscript>2+</superscript> ) in IAV endocytosis. We have found that IAV infection is accompanied by the increase in concentration of cytosolic Ca <superscript>2+</superscript> , which is mainly attributed to the influx of extracellular Ca <superscript>2+</superscript> . When Ca <superscript>2+</superscript> influx is abolished, IAV internalization will be markedly suppressed, but the virus attachment to its host cells will be unaffected. Extracellular Ca <superscript>2+</superscript> influx is essential to the clathrin-mediated endocytosis (CME) of IAVs but dispensable to the clathrin-independent endocytosis of the virus and is dispensable to the CME of transferrin or low-density lipoprotein as a control. Ca <superscript>2+</superscript> influx might participate in the dynamin-promoted membrane fission in the CME of IAVs. Our study highlights that IAVs enter host cells via extracellular Ca <superscript>2+</superscript> influx-involved clathrin- and dynamin-dependent endocytosis, which will facilitate better understanding of IAV infection and development of anti-influenza drugs.
Details
- Language :
- English
- ISSN :
- 2576-6422
- Volume :
- 4
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- ACS applied bio materials
- Publication Type :
- Academic Journal
- Accession number :
- 35014330
- Full Text :
- https://doi.org/10.1021/acsabm.0c00968