Back to Search
Start Over
Untargeted and Targeted LC-MS/MS Based Metabolomics Study on In Vitro Culture of Phaeoacremonium Species.
- Source :
-
Journal of fungi (Basel, Switzerland) [J Fungi (Basel)] 2022 Jan 06; Vol. 8 (1). Date of Electronic Publication: 2022 Jan 06. - Publication Year :
- 2022
-
Abstract
- Grapevine ( Vitis vinifera L.) can be affected by many different biotic agents, including tracheomycotic fungi such as Phaeomoniella chlamydospora and Phaeoacremonium minimum , which are the main causal agent of Esca and Petri diseases. Both fungi produce phytotoxic naphthalenone polyketides, namely scytalone and isosclerone, that are related to symptom development. The main objective of this study was to investigate the secondary metabolites produced by three Phaeoacremonium species and to assess their phytotoxicity by in vitro bioassay. To this aim, untargeted and targeted LC-MS/MS-based metabolomics were performed. High resolution mass spectrometer UHPLC-Orbitrap was used for the untargeted profiling and dereplication of secondary metabolites. A sensitive multi reaction monitoring (MRM) method for the absolute quantification of scytalone and isosclerone was developed on a UPLC-QTrap. Different isolates of P. italicum , P. alvesii and P. rubrigenum were grown in vitro and the culture filtrates and organic extracts were assayed for phytotoxicity. The toxic effects varied within and among fungal isolates. Isosclerone and scytalone were dereplicated by matching retention times and HRMS and MS/MS data with pure standards. The amount of scytalone and isosclerone differed within and among fungal species. To our best knowledge, this is the first study that applies an approach of LC-MS/MS-based metabolomics to investigate differences in the metabolic composition of organic extracts of Phaeoacremonium species culture filtrates.
Details
- Language :
- English
- ISSN :
- 2309-608X
- Volume :
- 8
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of fungi (Basel, Switzerland)
- Publication Type :
- Academic Journal
- Accession number :
- 35049995
- Full Text :
- https://doi.org/10.3390/jof8010055