Back to Search
Start Over
Specificity protein 1-induced serine peptidase inhibitor, Kunitz Type 1 antisense RNA1 regulates colorectal cancer cell proliferation, migration, invasion and apoptosis through targeting heparin binding growth factor via sponging microRNA-214.
- Source :
-
Bioengineered [Bioengineered] 2022 Feb; Vol. 13 (2), pp. 3309-3322. - Publication Year :
- 2022
-
Abstract
- Previous studies indicated that long noncoding RNA (lncRNA) serine peptidase inhibitor, Kunitz type 1 antisense RNA1 (SPINT1-AS1) could function as an oncogenic gene in various human cancers. However, the regulatory mechanisms of SPINT1-AS1 in the tumorigenesis of colorectal cancer (CRC) remain unclear. It was found that SPINT1-AS1 was upregulated in CRC and contributed to the poor prognosis of CRC patients. Silencing of SPINT1-AS1 inhibited proliferation and metastasis but increased apoptosis of CRC cells. Furthermore, we found that SP1 could activate SPINT1-AS1 by acting as a transcription factor. Meanwhile, we identified miR-214 was negatively regulated by SPINT1-AS1. Furthermore, miR-214 repression restored the suppressive effects on malignant biological behaviors of CRC caused by SPINT1-AS1 silencing. In addition, SPINT1-AS1 mediated HDGF expression through targeting miR-214. Finally, overexpressed heparin-binding growth factor (HDGF) overturned the effects on viability, metastasis, and apoptosis of CRC cells induced by SPINT1-AS1 depletion or miR-214 upregulation. In conclusion, our results demonstrated that SP1-induced SPINT1-AS1 could facilitate CRC progression by inhibiting miR-214 and increasing HDGF expression. These findings might provide a new approach for CRC treatment.
- Subjects :
- Aged
Caco-2 Cells
Colorectal Neoplasms genetics
Female
HCT116 Cells
Humans
Male
MicroRNAs genetics
Middle Aged
Neoplasm Invasiveness
Neoplasm Proteins genetics
RNA, Antisense genetics
RNA, Neoplasm genetics
Apoptosis
Cell Movement
Cell Proliferation
Colorectal Neoplasms metabolism
MicroRNAs metabolism
Neoplasm Proteins metabolism
RNA, Antisense metabolism
RNA, Neoplasm metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 2165-5987
- Volume :
- 13
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Bioengineered
- Publication Type :
- Academic Journal
- Accession number :
- 35068341
- Full Text :
- https://doi.org/10.1080/21655979.2022.2026859