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Developing a Microbubble-Based Contrast Agent for Synchrotron Multiple-Image Radiography.
- Source :
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Molecular imaging and biology [Mol Imaging Biol] 2022 Aug; Vol. 24 (4), pp. 590-599. Date of Electronic Publication: 2022 Feb 08. - Publication Year :
- 2022
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Abstract
- Purpose: Multiple-image radiography (MIR) is an analyzer-based synchrotron X-ray imaging approach capable of dissociating absorption, refraction, and scattering components of X-ray interaction with the material. It generates additional image contrast mechanisms (besides absorption), especially in the case of soft tissues, while minimizing absorbed radiation dose. Our goal is to develop a contrast agent for MIR using ultrasound microbubbles by carrying out a systematic assessment of size, shell material, and concentration.<br />Procedures: Microbubbles were synthesized with two different shell materials: phospholipid and polyvinyl-alcohol. Polydisperse perfluorobutane-filled lipid microbubbles were divided into five size groups using centrifugation. Two distributions of air-filled polymer microbubbles were generated: 2-3 µm and 3-4 µm. A subset of polymer microbubbles 3-4 µm had iron oxide nanoparticles incorporated into their shell or coated on their surface. Microbubbles were immobilized in agar with different concentrations: 5 × 10 <superscript>7</superscript> , 5 × 10 <superscript>6</superscript> , and 5 × 10 <superscript>5</superscript>  MBs/ml. MIR was conducted on the BioMedical Imaging and Therapy beamline at the Canadian Light Source. Three images were generated: Gaussian amplitude, refraction, and ultra-small-angle X-ray scattering (USAXS). The contrast signal was quantified by measuring mean pixel values and comparing them with agar.<br />Results: No difference was detected in absorption or refraction images of all tested microbubbles. Using USAXS, a significant signal increase was observed with lipid microbubbles 6-10 µm at the highest concentration (p = 0.02), but no signal was observed at lower concentrations.<br />Conclusions: These data indicate that lipid microbubbles 6-10 µm are candidates as contrast agents for MIR, specifically for USAXS. A minimum concentration of 5 × 10 <superscript>7</superscript> microbubbles (lipid-shell 6-10 µm) per milliliter was needed to generate a detectable signal.<br /> (© 2022. World Molecular Imaging Society.)
- Subjects :
- Agar
Canada
Lipids
Polymers
Radiography
Synchrotrons
Contrast Media
Microbubbles
Subjects
Details
- Language :
- English
- ISSN :
- 1860-2002
- Volume :
- 24
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Molecular imaging and biology
- Publication Type :
- Academic Journal
- Accession number :
- 35137326
- Full Text :
- https://doi.org/10.1007/s11307-022-01705-5