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Long non-coding RNA (lncRNA) plasmacytoma variant translocation 1 gene (PVT1) modulates the proliferation and apoptosis of acute lymphoblastic leukemia cells by sponging miR-486-5p.
- Source :
-
Bioengineered [Bioengineered] 2022 Feb; Vol. 13 (2), pp. 4587-4597. - Publication Year :
- 2022
-
Abstract
- Long non-coding RNA (lncRNA) plasmacytoma variant translocation 1 gene (PVT1) is related to the progress of various cancers. Here, we illuminated the role of PVT1 in acute lymphoblastic leukemia (ALL) cell proliferation and apoptosis. PVT1 was upregulated in plasma samples from patients with ALL and ALL cell lines. PVT1 silencing repressed cell viability and enhanced cell apoptosis in Jurkat and SUP-B15 cells. PVT1 targeted microRNA-486-5p (miR-486-5p) and negatively modulated miR-486-5p expression. Upregulation of miR-486-5p decreased cell viability and increased ALL cell apoptosis. Mastermind Like Transcriptional Coactivator 3 (MAML3) was a downstream molecule of miR-486-5p and miR-486-5p mimic transfection weakened its expression in ALL cells. Rescue experiments proved that reintroduction of PVT1 counteracted the impacts of miR-486-5p in ALL cell proliferation and apoptosis. In vivo , PVT1 silencing repressed the tumor growth of SUP-B15 cells and reduced the expression of MAML3. Altogether, silencing of PVT1 inhibited ALL cell growth and induced cell apoptosis through sponging miR-486-5p.
- Subjects :
- Animals
Cell Line, Tumor
Humans
Mice
Mice, Nude
Trans-Activators genetics
Apoptosis genetics
Cell Proliferation genetics
MicroRNAs genetics
Precursor Cell Lymphoblastic Leukemia-Lymphoma genetics
Precursor Cell Lymphoblastic Leukemia-Lymphoma metabolism
Precursor Cell Lymphoblastic Leukemia-Lymphoma pathology
RNA, Long Noncoding genetics
Subjects
Details
- Language :
- English
- ISSN :
- 2165-5987
- Volume :
- 13
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Bioengineered
- Publication Type :
- Academic Journal
- Accession number :
- 35152842
- Full Text :
- https://doi.org/10.1080/21655979.2022.2031405