Back to Search
Start Over
Combined Transcriptomics and Metabolomics Analysis Reveals the Molecular Mechanism of Salt Tolerance of Huayouza 62, an Elite Cultivar in Rapeseed ( Brassica napus L.).
- Source :
-
International journal of molecular sciences [Int J Mol Sci] 2022 Jan 24; Vol. 23 (3). Date of Electronic Publication: 2022 Jan 24. - Publication Year :
- 2022
-
Abstract
- Soil salinity is one of the most significant abiotic stresses affecting crop yield around the world. To explore the molecular mechanism of salt tolerance in rapeseed ( Brassica napus L.), the transcriptome analysis and metabolomics analysis were used to dissect the differentially expressed genes and metabolites in two rapeseed varieties with significant differences in salt tolerance; one is an elite rapeseed cultivar, Huayouza 62. A total of 103 key differentially expressed metabolites (DEMs) and 53 key differentials expressed genes (DEGs) that might be related to salt stress were identified through metabolomics and transcriptomics analysis. GO and KEGG analysis revealed that the DEGs were mainly involved in ion transport, reactive oxygen scavenging, osmotic regulation substance synthesis, and macromolecular protein synthesis. The DEMs were involved in TCA cycle, proline metabolism, inositol metabolism, carbohydrate metabolic processes, and oxidation-reduction processes. In addition, overexpression of BnLTP3 , which was one of the key DEGs, could increase tolerance to salt stress in Arabidopsis plants. This study reveals that the regulation mechanism of salt tolerance in rapeseed at the transcriptome and metabolism level and provides abundant data for further in-depth identification of essential salt tolerance genes.
- Subjects :
- China
Gene Expression genetics
Gene Expression Profiling methods
Gene Expression Regulation, Plant genetics
Metabolomics methods
Salt Stress genetics
Salt Tolerance physiology
Stress, Physiological genetics
Transcriptome genetics
Brassica napus genetics
Brassica napus metabolism
Salt Tolerance genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1422-0067
- Volume :
- 23
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- International journal of molecular sciences
- Publication Type :
- Academic Journal
- Accession number :
- 35163202
- Full Text :
- https://doi.org/10.3390/ijms23031279