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Salicylic Acid Is Required for Broad-Spectrum Disease Resistance in Rice.
- Source :
-
International journal of molecular sciences [Int J Mol Sci] 2022 Jan 25; Vol. 23 (3). Date of Electronic Publication: 2022 Jan 25. - Publication Year :
- 2022
-
Abstract
- Rice plants contain high basal levels of salicylic acid (SA), but some of their functions remain elusive. To elucidate the importance of SA homeostasis in rice immunity, we characterized four rice SA hydroxylase genes ( OsSAHs ) and verified their roles in SA metabolism and disease resistance. Recombinant OsSAH proteins catalyzed SA in vitro, while OsSAH3 protein showed only SA 5-hydroxylase (SA5H) activity, which was remarkably higher than that of other OsSAHs that presented both SA3H and SA5H activities. Amino acid substitutions revealed that three amino acids in the binding pocket affected SAH enzyme activity and/or specificity. Knockout OsSAH 2 and OsSAH3 ( sah KO) genes conferred enhanced resistance to both hemibiotrophic and necrotrophic pathogens, whereas overexpression of each OsSAH gene increased susceptibility to the pathogens. sah KO mutants showed increased SA and jasmonate levels compared to those of the wild type and OsSAH -overexpressing plants. Analysis of the OsSAH3 promoter indicated that its induction was mainly restricted around Magnaporthe oryzae infection sites. Taken together, our findings indicate that SA plays a vital role in immune signaling. Moreover, fine-tuning SA homeostasis through suppression of SA metabolism is an effective approach in studying broad-spectrum disease resistance in rice.
- Subjects :
- China
Cyclopentanes
Dioxygenases
Gene Expression genetics
Gene Expression Regulation, Plant drug effects
Gene Expression Regulation, Plant genetics
Hydroxylation
Oryza drug effects
Oryza metabolism
Oxylipins
Plant Diseases genetics
Plant Immunity drug effects
Plant Immunity physiology
Plant Proteins genetics
Plants, Genetically Modified metabolism
Salicylic Acid pharmacology
Disease Resistance physiology
Oryza genetics
Salicylic Acid metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1422-0067
- Volume :
- 23
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- International journal of molecular sciences
- Publication Type :
- Academic Journal
- Accession number :
- 35163275
- Full Text :
- https://doi.org/10.3390/ijms23031354