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Role of the Pbrm1 subunit and the PBAF complex in Schwann cell development.
- Source :
-
Scientific reports [Sci Rep] 2022 Feb 16; Vol. 12 (1), pp. 2651. Date of Electronic Publication: 2022 Feb 16. - Publication Year :
- 2022
-
Abstract
- Myelin sheath formation in the peripheral nervous system and the ensuing saltatory conduction rely on differentiated Schwann cells. We have previously shown that transition of Schwann cells from an immature into a differentiated state requires Brg1 that serves as the central energy generating subunit in two related SWI/SNF-type chromatin remodelers, the BAF and the PBAF complex. Here we used conditional deletion of Pbrm1 to selectively interfere with the PBAF complex in Schwann cells. Despite efficient loss of Pbrm1 early during lineage progression, we failed to detect any substantial alterations in the number, proliferation or survival of immature Schwann cells as well as in their rate and timing of terminal differentiation. As a consequence, postnatal myelin formation in peripheral nerves appeared normal. There were no inflammatory alterations in the nerve or other signs of a peripheral neuropathy. We conclude from our study that Pbrm1 and very likely the PBAF complex are dispensable for proper Schwann cell development and that Schwann cell defects previously observed upon Brg1 deletion are mostly attributable to altered or absent function of the BAF complex.<br /> (© 2022. The Author(s).)
- Subjects :
- Animals
Cell Lineage genetics
Cell Proliferation genetics
Cell Survival genetics
DNA Helicases genetics
Gene Deletion
Mice
Myelin Sheath physiology
Nuclear Proteins genetics
Peripheral Nerves physiology
Transcription Factors genetics
Cell Differentiation genetics
Chromosomal Proteins, Non-Histone physiology
DNA-Binding Proteins physiology
Schwann Cells physiology
Transcription Factors physiology
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 12
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 35173232
- Full Text :
- https://doi.org/10.1038/s41598-022-06588-8