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Caprylic Acid Improves Lipid Metabolism, Suppresses the Inflammatory Response and Activates the ABCA1/p-JAK2/p-STAT3 Signaling Pathway in C57BL/6J Mice and RAW264.7 Cells.
- Source :
-
Biomedical and environmental sciences : BES [Biomed Environ Sci] 2022 Feb 20; Vol. 35 (2), pp. 95-106. - Publication Year :
- 2022
-
Abstract
- Objective: This study aimed to investigate the effects of caprylic acid (C8:0) on lipid metabolism and inflammation, and examine the mechanisms underlying these effects in mice and cells.<br />Methods: Fifty-six 6-week-old male C57BL/6J mice were randomly allocated to four groups fed a high-fat diet (HFD) without or with 2% C8:0, palmitic acid (C16:0) or eicosapentaenoic acid (EPA). RAW246.7 cells were randomly divided into five groups: normal, lipopolysaccharide (LPS), LPS+C8:0, LPS+EPA and LPS+cAMP. The serum lipid profiles, inflammatory biomolecules, and ABCA1 and JAK2/STAT3 mRNA and protein expression were measured.<br />Results: C8:0 decreased TC and LDL-C, and increased the HDL-C/LDL-C ratio after injection of LPS. Without LPS, it decreased TC in mice ( P < 0.05). Moreover, C8:0 decreased the inflammatory response after LPS treatment in both mice and cells ( P < 0.05). Mechanistic investigations in C57BL/6J mouse aortas after injection of LPS indicated that C8:0 resulted in higher ABCA1 and JAK2/STAT3 expression than that with HFD, C16:0 and EPA, and resulted in lower TNF-α, NF-κB mRNA expression than that with HFD ( P < 0.05). In RAW 264.7 cells, C8:0 resulted in lower expression of pNF-κBP65 than that in the LPS group, and higher protein expression of ABCA1, p-JAK2 and p-STAT3 than that in the LPS and LPS+cAMP groups ( P < 0.05).<br />Conclusion: Our studies demonstrated that C8:0 may play an important role in lipid metabolism and the inflammatory response, and the mechanism may be associated with ABCA1 and the p-JAK2/p-STAT3 signaling pathway.<br /> (Copyright © 2022 The Editorial Board of Biomedical and Environmental Sciences. Published by China CDC. All rights reserved.)
- Subjects :
- ATP Binding Cassette Transporter 1 genetics
Animals
Caprylates chemistry
Cholesterol metabolism
Diet, High-Fat adverse effects
Humans
Inflammation etiology
Inflammation immunology
Inflammation metabolism
Janus Kinase 2 genetics
Macrophages immunology
Male
Mice
Mice, Inbred C57BL
RAW 264.7 Cells
STAT3 Transcription Factor genetics
Signal Transduction
ATP Binding Cassette Transporter 1 immunology
Caprylates administration & dosage
Inflammation drug therapy
Janus Kinase 2 immunology
Lipid Metabolism drug effects
Macrophages drug effects
STAT3 Transcription Factor immunology
Subjects
Details
- Language :
- English
- ISSN :
- 2214-0190
- Volume :
- 35
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Biomedical and environmental sciences : BES
- Publication Type :
- Academic Journal
- Accession number :
- 35197174
- Full Text :
- https://doi.org/10.3967/bes2022.014